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Electrostatic strain and concerted motions in the transient complex between plastocyanin and cytochrome f from the cyanobacterium Phormidium laminosum

 

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dc.creator Díaz Moreno, Irene es
dc.creator Muñoz López, Francisco Jesús es
dc.creator Frutos Beltrán, Estrella es
dc.creator Rosa Acosta, Miguel Ángel de la es
dc.creator Díaz Quintana, Antonio Jesús es
dc.date.accessioned 2019-03-27T09:43:52Z
dc.date.available 2019-03-27T09:43:52Z
dc.date.issued 2009
dc.identifier.citation Díaz Moreno, I., Muñoz López, F.J., Frutos Beltrán, E., Rosa Acosta, M.Á.d.l. y Díaz Quintana, A.J. (2009). Electrostatic strain and concerted motions in the transient complex between plastocyanin and cytochrome f from the cyanobacterium Phormidium laminosum. Bioelectrochemistry, 77 (1), 43-52.
dc.identifier.issn 1567-5394 es
dc.identifier.uri https://hdl.handle.net/11441/84781
dc.description.abstract Many fleeting macromolecular interactions, like those being involved in electron transport, are essential in biology. However, little is known about the behaviour of the partners and their dynamics within their shortlived complex. To tackle such issue, we have performed molecular dynamics simulations on an electron transfer complex formed by plastocyanin and cytochrome f from the cyanobacterium Phormidium laminosum. Besides simulations of the isolated partners, two independent trajectories of the complex were calculated, starting from the two different conformations in the NMR ensemble. The first one leads to a more stable ensemble with a shorter distance between the metal sites of the two partners. The second experiences a significant drift of the complex conformation. Analyses of the distinct calculations show that the conformation of cytochrome f is strained upon binding of its partner, and relaxes upon its release. Interestingly, the principal component analysis of the trajectories indicates that plastocyanin displays a concerted motion with the small domain of cytochrome f that can be attributed to electrostatic interactions between the two proteins. es
dc.description.sponsorship Ministerio de Ciencia e Innovación BFU2006-01361 BES-2005-10404 es
dc.description.sponsorship Junta de Andalucía BIO-198 P06-CVI-01713 es
dc.format application/pdf es
dc.language.iso eng es
dc.publisher Elsevier es
dc.relation.ispartof Bioelectrochemistry, 77 (1), 43-52.
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 Internacional *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/ *
dc.subject Cytochrome f es
dc.subject Domain motions es
dc.subject Molecular dynamics es
dc.subject Plastocyanin es
dc.subject Transient protein complexes es
dc.title Electrostatic strain and concerted motions in the transient complex between plastocyanin and cytochrome f from the cyanobacterium Phormidium laminosum es
dc.type info:eu-repo/semantics/article es
dc.type.version info:eu-repo/semantics/acceptedVersion es
dc.rights.accessrights info:eu-repo/semantics/openAccess es
dc.contributor.affiliation Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular es
dc.relation.projectID BFU2006-01361 es
dc.relation.projectID BES-2005-10404 es
dc.relation.projectID BIO-198 es
dc.relation.projectID P06-CVI-01713 es
dc.relation.publisherversion https://doi.org/10.1016/j.bioelechem.2009.06.003 es
dc.identifier.doi 10.1016/j.bioelechem.2009.06.003 es
idus.format.extent 10 p. es
dc.journaltitle Bioelectrochemistry es
dc.publication.volumen 77 es
dc.publication.issue 1 es
dc.publication.initialPage 43 es
dc.publication.endPage 52 es
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