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Ribosomal protein L14 contributes to the early assembly of 60S ribosomal subunits in Saccharomyces cerevisiae


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dc.creator Espinar Marchena, Francisco José es
dc.creator Rodríguez Galán, Olga es
dc.creator Fernández Fernández, José es
dc.creator Linnemann, Jan es
dc.creator Cruz Díaz, Jesús de la es 2018-12-20T12:41:59Z 2018-12-20T12:41:59Z 2018
dc.identifier.citation Espinar Marchena, F.J., Rodríguez Galán, O., Fernández Fernández, J., Linnemann, J. y Cruz Díaz, J.d.l. (2018). Ribosomal protein L14 contributes to the early assembly of 60S ribosomal subunits in Saccharomyces cerevisiae. Nucleic Acids Research, 46 (9), 4715-4732.
dc.identifier.issn 1362-4962 es
dc.description.abstract The contribution of most ribosomal proteins to ribo- some synthesis has been quite well analysed in Sac- charomyces cerevisiae . However, few yeast riboso- mal proteins still await characterization. Herein, we show that L14, an essential 60S ribosomal protein, assembles in the nucleolus at an early stage into pre- 60S particles. Depletion of L14 results in a deficit in 60S subunits and defective processing of 27SA 2 and 27SA 3 to 27SB pre-rRNAs. As a result, 27S pre-rRNAs are subjected to turnover and export of pre-60S par- ticles is blocked. These phenotypes likely appear as the direct consequence of the reduced pre-60S par- ticle association not only of L14 upon its depletion but also of a set of neighboring ribosomal proteins located at the solvent interface of 60S subunits and the adjacent region surrounding the polypeptide exit tunnel. These pre-60S intermediates also lack some essential trans -acting factors required for 27SB pre- rRNA processing but accumulate practically all fac- tors required for processing of 27SA 3 pre-rRNA. We have also analysed the functional interaction be- tween the eukaryote-specific carboxy-terminal exten- sions of the neighboring L14 and L16 proteins. Our results indicate that removal of the most distal parts of these extensions cause slight translation alter- ations in mature 60S subunits. es
dc.description.sponsorship Ministerio de Economía y Competitividad (BFU2013-42958-P), (BFU2016-75352- P) es
dc.description.sponsorship Deutsche Forschungs- gemeinschaft’ (SFB 960) es
dc.format application/pdf es
dc.language.iso eng es
dc.publisher Oxford University Press es
dc.relation.ispartof Nucleic Acids Research, 46 (9), 4715-4732.
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 Internacional *
dc.rights.uri *
dc.title Ribosomal protein L14 contributes to the early assembly of 60S ribosomal subunits in Saccharomyces cerevisiae es
dc.type info:eu-repo/semantics/article es
dc.type.version info:eu-repo/semantics/publishedVersion es
dc.rights.accessrights info:eu-repo/semantics/openAccess es
dc.contributor.affiliation Universidad de Sevilla. Departamento de Genética es
dc.relation.projectID BFU2013-42958-P es
dc.relation.projectID BFU2016-75352- P es
dc.relation.projectID SFB 960 es
dc.relation.publisherversion es
dc.identifier.doi 10.1093/nar/gky123 es
idus.format.extent 17 es
dc.journaltitle Nucleic Acids Research es
dc.publication.volumen 46 es
dc.publication.issue 9 es
dc.publication.initialPage 4715 es
dc.publication.endPage 4732 es
dc.contributor.funder Ministerio de Economía y Competitividad (MINECO). España
dc.contributor.funder Deutsche Forschungsgemeinschaft / German Research Foundation (DFG)
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