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STEM-in-SEM high resolution imaging of gold nanoparticles and bivalve tissues in bioaccumulation experiments

 

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dc.creator García Negrete, Carlos Andrés es
dc.creator Jiménez de Haro, María del Carmen es
dc.creator Blasco, Javier es
dc.creator Soto, M. es
dc.creator Fernández Camacho, María Asunción es
dc.date.accessioned 2018-12-03T16:32:01Z
dc.date.available 2018-12-03T16:32:01Z
dc.date.issued 2015
dc.identifier.citation García Negrete, C.A., Jiménez de Haro, M.d.C., Blasco, J., Soto, M. y Fernández Camacho, M.A. (2015). STEM-in-SEM high resolution imaging of gold nanoparticles and bivalve tissues in bioaccumulation experiments. Analyst, 140, 3082-3089.
dc.identifier.issn 0003-2654 es
dc.identifier.issn 1364-5528 es
dc.identifier.uri https://hdl.handle.net/11441/80755
dc.description.abstract The methodology termed scanning transmission electron microscopy in scanning electron microscopy (STEM-in-SEM) has been used in this work to study the uptake of citrate stabilized gold nanoparticles (AuNPs) (average particle sizes of 23.5 ± 4.0 nm) into tissue samples upon in vitro exposure of the dissected gills of the Ruditapes philippinarum marine bivalve to the nanoparticle suspensions. The STEM-in- SEM methodology has been optimized for achieving optimum resolution under SEM low voltage operating conditions (20–30 kV). Based on scanning microscope assessments and resolution testing (SMART), resolutions well below 10 nm were appropriately achieved by working at magnifications over 100k×, with experimental sample thickness between 300 and 200 nm. These relatively thick slices appear to be stable under the beam and help avoid NP displacement during cutting. We herein show that both localizing of the internalized nanoparticles and imaging of ultrastructural disturbances in gill tissues are strongly accessible due to the improved resolution, even at sample thicknesses higher than those normally employed in standard TEM techniques at higher voltages. Ultrastructural imaging of bio-nano features in bioaccumulation experiments have been demonstrated in this study. es
dc.description.sponsorship Junta de Andalucía PE2009-FQM-4554, PE2011-RNM-7812, TEP-217 es
dc.description.sponsorship European Union CT-REGPOT2011-1-285895 es
dc.format application/pdf es
dc.language.iso eng es
dc.publisher Royal Society of Chemistry (Great Britain) es
dc.relation.ispartof Analyst, 140, 3082-3089.
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 Internacional *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/ *
dc.title STEM-in-SEM high resolution imaging of gold nanoparticles and bivalve tissues in bioaccumulation experiments es
dc.type info:eu-repo/semantics/article es
dc.type.version info:eu-repo/semantics/publishedVersion es
dc.rights.accessrights info:eu-repo/semantics/openAccess es
dc.relation.projectID PE2009-FQM-4554 es
dc.relation.projectID PE2011-RNM-7812 es
dc.relation.projectID TEP-217 es
dc.relation.projectID CT-REGPOT2011-1-285895 es
dc.relation.publisherversion http://dx.doi.org/10.1039/c4an01643b es
dc.identifier.doi 10.1039/c4an01643b es
idus.format.extent 8 p. es
dc.journaltitle Analyst es
dc.publication.volumen 140 es
dc.publication.initialPage 3082 es
dc.publication.endPage 3089 es
dc.contributor.funder Junta de Andalucía
dc.contributor.funder European Union (UE)
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