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Effective Knockdown of Gene Expression in Primary Microglia With siRNA and Magnetic Nanoparticles Without Cell Death or Inflammation

 

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dc.creator Carrillo Jiménez, Alejandro es
dc.creator Puigdellívol, Mar es
dc.creator Vilalta, Anna es
dc.creator Venero Recio, José Luis es
dc.creator Brown, Charles Guy es
dc.creator Burguillos García, Miguel Ángel es
dc.date.accessioned 2018-10-16T09:20:37Z
dc.date.available 2018-10-16T09:20:37Z
dc.date.issued 2018
dc.identifier.citation Carrillo Jiménez, A., Puigdellívol, M., Vilalta, ., Venero Recio, J.L., Brown, C.G. y Burguillos García, M.Á. (2018). Effective Knockdown of Gene Expression in Primary Microglia With siRNA and Magnetic Nanoparticles Without Cell Death or Inflammation. Frontiers in Cellular Neuroscience, 12 (313), 1-8.
dc.identifier.issn 1662-5102 es
dc.identifier.uri https://hdl.handle.net/11441/79435
dc.description.abstract Microglia, the resident immune cells of the brain, have multiple functions in physiological and pathological conditions, including Alzheimer’s disease (AD). The use of primary microglial cell cultures has proved to be a valuable tool to study microglial biology under various conditions. However, more advanced transfection methodologies for primary cultured microglia are still needed, as current methodologies provide low transfection efficiency and induce cell death and/or inflammatory activation of the microglia. Here, we describe an easy, and effective method based on the Glial-Mag method (OZ Biosciences) using magnetic nanoparticles and a magnet to successfully transfect primary microglia cells with different small interfering RNAs (siRNAs). This method does not require specialist facilities or specific training and does not induce cell toxicity or inflammatory activation. We demonstrate that this protocol successfully decreases the expression of two key genes associated with AD, the triggering receptor expressed in myeloid cells 2 (TREM2) and CD33, in primary microglia cell cultures. es
dc.description.sponsorship España, Ministerio de Educación, Cultura y Deporte a pre-doctoral fellowship es
dc.format application/pdf es
dc.language.iso eng es
dc.publisher Frontiers Media es
dc.relation.ispartof Frontiers in Cellular Neuroscience, 12 (313), 1-8.
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 Internacional *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/ *
dc.subject microglia es
dc.subject siRNA es
dc.subject transfection es
dc.subject TREM2 es
dc.subject CD33 es
dc.subject Alzheimer’s disease es
dc.title Effective Knockdown of Gene Expression in Primary Microglia With siRNA and Magnetic Nanoparticles Without Cell Death or Inflammation es
dc.type info:eu-repo/semantics/article es
dc.type.version info:eu-repo/semantics/publishedVersion es
dc.rights.accessrights info:eu-repo/semantics/openAccess es
dc.contributor.affiliation Universidad de Sevilla. Departamento de Bioquímica y Biología Molecular es
dc.relation.publisherversion http://dx.doi.org/10.3389/fncel.2018.00313 es
dc.identifier.doi 10.3389/fncel.2018.00313 es
idus.format.extent 8 p. es
dc.journaltitle Frontiers in Cellular Neuroscience es
dc.publication.volumen 12 es
dc.publication.issue 313 es
dc.publication.initialPage 1 es
dc.publication.endPage 8 es
dc.contributor.funder Ministerio de Educación, Cultura y Deporte (MECD). España
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