Opened Access Detection of DNA-RNA hybrids in vivo

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Author: García Rubio, María
Barroso Ceballos, Sonia Inés
Aguilera López, Andrés
Department: Universidad de Sevilla. Departamento de Genética
Date: 2018
Published in: Methods in Molecular Biology
ISBN/ISSN: 1064-3745
Document type: Chapter of Book
Abstract: DNA-RNA hybrids form naturally during essential cellular functions such as transcription and replication. However, they may be an important source of genome instability, a hallmark of cancer and genetic diseases. Detection of DNA-RNA hybrids in cells is becoming crucial to understand an increasing number of molecular biology processes in genome dynamics and function and to identify new factors and mechanisms responsible for disease in biomedical research. Here, we describe two different procedures for the reliable detection of DNA-RNA hybrids in the yeast Saccharomyces cerevisiae and in human cells: DNA-RNA Immunoprecipitation (DRIP) and Immunofluorescence.
Cite: García Rubio, M., Barroso Ceballos, S.I., y Aguilera López, A. (2018). Detection of DNA-RNA hybrids in vivo. En Methods in Molecular Biology (pp. 347-361). Humana Press Inc.
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DOI: 10.1007/978-1-4939-7306-4_24

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