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Intracellular Trafficking and Persistence of Acinetobacter baumannii Requires Transcription Factor EB

 

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Opened Access Intracellular Trafficking and Persistence of Acinetobacter baumannii Requires Transcription Factor EB
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Author: Parra Millán, Raquel
Guerrero Gómez, David
Ayerbe Algaba, Rafael
Pachón Ibáñez, María Eugenia
Miranda Vizuete, Antonio
Pachón Díaz, Jerónimo
Smani, Younes
Department: Instituto de Biomedicina de Sevilla (IBIS)
Date: 2018-03-28
Published in: mSphere, 3 (2), 00106-18-1-00106-18-14.
Document type: Article
Abstract: Acinetobacter baumannii is a significant human pathogen associated with hospital-acquired infections. While adhesion, an initial and important step in A. baumannii infection, is well characterized, the intracellular trafficking of this pathogen inside host cells remains poorly studied. Here, we demonstrate that transcription factor EB (TFEB) is activated after A. baumannii infection of human lung epithelial cells (A549). We also show that TFEB is required for the invasion and persistence inside A549 cells. Consequently, lysosomal biogenesis and autophagy activation were observed after TFEB activation which could increase the death of A549 cells. In addition, using the Caenorhabditis elegans infection model by A. baumannii, the TFEB orthologue HLH-30 was required for survival of the nematode to infection, although nuclear translocation of HLH-30 was not required. These results identify TFEB as a conserved key factor in the pathogenesis of A. baumannii.
Cite: Parra Millán, R., Guerrero Gómez, D., Ayerbe Algaba, R., Pachón Ibáñez, M.E., Miranda Vizuete, A., Pachón Díaz, J. y Smani, Y. (2018). Intracellular Trafficking and Persistence of Acinetobacter baumannii Requires Transcription Factor EB. mSphere, 3 (2), 00106-18-1-00106-18-14.
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URI: https://hdl.handle.net/11441/75156

DOI: 10.1128/mSphere.00106-18

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