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Genetic and biochemical study of threonine-overproducing mutants of Saccharomyces cerevisiae

Opened Access Genetic and biochemical study of threonine-overproducing mutants of Saccharomyces cerevisiae

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Autor: Delgado, Marco A.
Guerrero, Josefa
Conde, Jaime
Departamento: Universidad de Sevilla. Departamento de Microbiología
Fecha: 1982
Publicado en: Molecular and Cellular Biology, 2 (7), 731-736.
Tipo de documento: Artículo
Resumen: Three threonine-overproducing mutants were obtained as prototrophic revertants of a hom3 mutant strain of Saccharomyces cerevisiae. The gene HOM3 codes for aspartokinase (aspartate kinase; EC 2.7.2.4), the first enzyme of the threonine-methionine biosynthetic route, which is subjected to feedback inhibition by threonine. Enzymatic studies indicated that aspartokinase from the revertants has lost the feedback inhibition, resulting in overproduction of threonine. These revertants also bore one or two additional mutations, named texl-J and tex2-1, which alone or jointly made possible the excretion of the threonine accumulated. The effect of these two genes on excretion is potentiated by excess inositol in the medium.
Cita: Delgado, M.A., Guerrero, J. y Conde, J. (1982). Genetic and biochemical study of threonine-overproducing mutants of Saccharomyces cerevisiae. Molecular and Cellular Biology, 2 (7), 731-736.
Tamaño: 909.0Kb
Formato: PDF

URI: https://hdl.handle.net/11441/70789

DOI: 10.1128/MCB.2.7.731

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