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A gene-specific requirement for FACT during transcription is related to the chromatin organization of the transcribed region

Opened Access A gene-specific requirement for FACT during transcription is related to the chromatin organization of the transcribed region

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Autor: Jimeno González, Silvia
Gómez Herrero, Fernando
Alepuz, Paula M.
Chávez de Diego, Sebastián
Departamento: Universidad de Sevilla. Departamento de Genética
Fecha: 2006
Publicado en: Molecular and Cellular Biology, 26 (23), 8710-8721.
Tipo de documento: Artículo
Resumen: The FACT complex stimulates transcription elongation on nucleosomal templates. In vivo experiments also involve FACT in the reassembly of nucleosomes traversed by RNA polymerase II. Since several features of chromatin organization vary throughout the genome, we wondered whether FACT is equally required for all genes. We show in this study that the in vivo depletion of Spt16, one of the subunits of Saccharomyces cerevisiae FACT, strongly affects transcription of three genes, GAL1, PHO5, and Kluyveromyces lactis LAC4, which exhibit positioned nucleosomes at their transcribed regions. In contrast, showing a random nucleosome structure, YAT1 and Escherichia coli lacZ are only mildly influenced by Spt16 depletion. We also show that the effect of Spt16 depletion on GAL1 expression is suppressed by a histone mutation and that the insertion of a GAL1 fragment, which allows the positioning of two nucleosomes, at the 5′ end of YAT1 makes the resulting transcription unit sensitive to Spt16 deple...
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Cita: Jimeno González, S., Gómez Herrero, F., Alepuz, P.M. y Chavez de Diego, S. (2006). A gene-specific requirement for FACT during transcription is related to the chromatin organization of the transcribed region. Molecular and Cellular Biology, 26 (23), 8710-8721.
Tamaño: 469.8Kb
Formato: PDF

URI: https://hdl.handle.net/11441/70526

DOI: 10.1128/MCB.01129-06

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