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dc.creatorLafuente Barquero, Juanes
dc.creatorLuke Glaser, Sarahes
dc.creatorGraf, Marcoes
dc.creatorSilva, Soniaes
dc.creatorGómez González, Belénes
dc.creatorAguilera López, Andréses
dc.date.accessioned2018-02-09T12:41:43Z
dc.date.available2018-02-09T12:41:43Z
dc.date.issued2017
dc.identifier.citationLafuente Barquero, J., Luke Glaser, ., Graf, M., Silva, S., Gómez González, B. y Aguilera López, A. (2017). The Smc5/6 complex regulates the yeast Mph1 helicase at RNA-DNA hybrid-mediated DNA damage. PLoS Genetics, 13 (12), 1-25.
dc.identifier.issn1553-7404)es
dc.identifier.urihttps://hdl.handle.net/11441/70178
dc.description.abstractRNA-DNA hybrids are naturally occurring obstacles that must be overcome by the DNA replication machinery. In the absence of RNase H enzymes, RNA-DNA hybrids accumulate, resulting in replication stress, DNA damage and compromised genomic integrity. We demonstrate that Mph1, the yeast homolog of Fanconi anemia protein M (FANCM), is required for cell viability in the absence of RNase H enzymes. The integrity of the Mph1 helicase domain is crucial to prevent the accumulation of RNA-DNA hybrids and RNA-DNA hybrid-dependent DNA damage, as determined by Rad52 foci. Mph1 forms foci when RNA-DNA hybrids accumulate, e.g. in RNase H or THO-complex mutants and at short telomeres. Mph1, however is a double-edged sword, whose action at hybrids must be regulated by the Smc5/6 complex. This is underlined by the observation that simultaneous inactivation of RNase H2 and Smc5/6 results in Mph1-dependent synthetic lethality, which is likely due to an accumulation of toxic recombination intermediates. The data presented here support a model, where Mph1’s helicase activity plays a crucial role in responding to persistent RNA-DNA hybrids.es
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherPublic Library of Sciencees
dc.relation.ispartofPLoS Genetics, 13 (12), 1-25.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleThe Smc5/6 complex regulates the yeast Mph1 helicase at RNA-DNA hybrid-mediated DNA damagees
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessrightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Genéticaes
dc.relation.publisherversionhttp://dx.doi.org/10.1371/journal.pgen.1007136es
dc.identifier.doi10.1371/journal.pgen.1007136es
idus.format.extent25 p.es
dc.journaltitlePLoS Geneticses
dc.publication.volumen13es
dc.publication.issue12es
dc.publication.initialPage1es
dc.publication.endPage25es

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