dc.creator | Lafuente Barquero, Juan | es |
dc.creator | Luke Glaser, Sarah | es |
dc.creator | Graf, Marco | es |
dc.creator | Silva, Sonia | es |
dc.creator | Gómez González, Belén | es |
dc.creator | Aguilera López, Andrés | es |
dc.date.accessioned | 2018-02-09T12:41:43Z | |
dc.date.available | 2018-02-09T12:41:43Z | |
dc.date.issued | 2017 | |
dc.identifier.citation | Lafuente Barquero, J., Luke Glaser, ., Graf, M., Silva, S., Gómez González, B. y Aguilera López, A. (2017). The Smc5/6 complex regulates the yeast Mph1 helicase at RNA-DNA hybrid-mediated DNA damage. PLoS Genetics, 13 (12), 1-25. | |
dc.identifier.issn | 1553-7404) | es |
dc.identifier.uri | https://hdl.handle.net/11441/70178 | |
dc.description.abstract | RNA-DNA hybrids are naturally occurring obstacles that must be overcome by the DNA replication machinery. In the absence of RNase H enzymes, RNA-DNA hybrids accumulate, resulting in replication stress, DNA damage and compromised genomic integrity. We demonstrate that Mph1, the yeast homolog of Fanconi anemia protein M (FANCM), is required for cell viability in the absence of RNase H enzymes. The integrity of the Mph1 helicase domain is crucial to prevent the accumulation of RNA-DNA hybrids and RNA-DNA hybrid-dependent DNA damage, as determined by Rad52 foci. Mph1 forms foci when RNA-DNA hybrids accumulate, e.g. in RNase H or THO-complex mutants and at short telomeres. Mph1, however is a double-edged sword, whose action at hybrids must be regulated by the Smc5/6 complex. This is underlined by the observation that simultaneous inactivation of RNase H2 and Smc5/6 results in Mph1-dependent synthetic lethality, which is likely due to an accumulation of toxic recombination intermediates. The data presented here support a model, where Mph1’s helicase activity plays a crucial role in responding to persistent RNA-DNA hybrids. | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | Public Library of Science | es |
dc.relation.ispartof | PLoS Genetics, 13 (12), 1-25. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | The Smc5/6 complex regulates the yeast Mph1 helicase at RNA-DNA hybrid-mediated DNA damage | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessrights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Genética | es |
dc.relation.publisherversion | http://dx.doi.org/10.1371/journal.pgen.1007136 | es |
dc.identifier.doi | 10.1371/journal.pgen.1007136 | es |
idus.format.extent | 25 p. | es |
dc.journaltitle | PLoS Genetics | es |
dc.publication.volumen | 13 | es |
dc.publication.issue | 12 | es |
dc.publication.initialPage | 1 | es |
dc.publication.endPage | 25 | es |