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The Smc5/6 complex regulates the yeast Mph1 helicase at RNA-DNA hybrid-mediated DNA damage

Opened Access The Smc5/6 complex regulates the yeast Mph1 helicase at RNA-DNA hybrid-mediated DNA damage

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Autor: Lafuente Barquero, Juan
Luke Glaser, Sarah
Graf, Marco
Silva, Sonia
Gómez González, Belén
Aguilera López, Andrés
Departamento: Universidad de Sevilla. Departamento de Genética
Fecha: 2017
Publicado en: PLoS Genetics, 13 (12), 1-25.
Tipo de documento: Artículo
Resumen: RNA-DNA hybrids are naturally occurring obstacles that must be overcome by the DNA replication machinery. In the absence of RNase H enzymes, RNA-DNA hybrids accumulate, resulting in replication stress, DNA damage and compromised genomic integrity. We demonstrate that Mph1, the yeast homolog of Fanconi anemia protein M (FANCM), is required for cell viability in the absence of RNase H enzymes. The integrity of the Mph1 helicase domain is crucial to prevent the accumulation of RNA-DNA hybrids and RNA-DNA hybrid-dependent DNA damage, as determined by Rad52 foci. Mph1 forms foci when RNA-DNA hybrids accumulate, e.g. in RNase H or THO-complex mutants and at short telomeres. Mph1, however is a double-edged sword, whose action at hybrids must be regulated by the Smc5/6 complex. This is underlined by the observation that simultaneous inactivation of RNase H2 and Smc5/6 results in Mph1-dependent synthetic lethality, which is likely due to an accumulation of toxic recombination intermediates. Th...
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Cita: Lafuente Barquero, J., Luke Glaser, ., Graf, M., Silva, S., Gómez González, B. y Aguilera López, A. (2017). The Smc5/6 complex regulates the yeast Mph1 helicase at RNA-DNA hybrid-mediated DNA damage. PLoS Genetics, 13 (12), 1-25.
Tamaño: 6.249Mb
Formato: PDF

URI: https://hdl.handle.net/11441/70178

DOI: 10.1371/journal.pgen.1007136

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