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Identification of a promising multivalent inhibitor of the DC-SIGN dependent uptake of HIV-1 and Dengue virus

 

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dc.creator Varga, Norbert es
dc.creator Ribeiro Viana, Renato es
dc.creator Berzi, Angela es
dc.creator Ramdasi, Rasika es
dc.creator Daghetti, Anna es
dc.creator Vettoretti, Gerolamo es
dc.creator Amara, Ali es
dc.creator ClericI, Mario es
dc.creator Rojo Marcos, Francisco Javier es
dc.creator Fieschi, Franck es
dc.creator Bernardi, Anna es
dc.date.accessioned 2018-02-02T14:45:06Z
dc.date.available 2018-02-02T14:45:06Z
dc.date.issued 2014
dc.identifier.citation Varga, N., Ribeiro Viana, R., Berzi, A., Ramdasi, R., Daghetti, A., Vettoretti, G.,...,Bernardi, A. (2014). Identification of a promising multivalent inhibitor of the DC-SIGN dependent uptake of HIV-1 and Dengue virus. Biomaterials, 35 (13), 4175-4184.
dc.identifier.issn 0142-9612 es
dc.identifier.uri https://hdl.handle.net/11441/69933
dc.description.abstract DC-SIGN is a C-type lectin receptor on antigen presenting cells (dendritic cells) which has an important role in some viral infection, notably by HIV and Dengue virus (DV). Multivalent presentation of carbohydrates on dendrimeric scaffolds has been shown to inhibit DC-SIGN binding to HIV envelope glycoprotein gp120, thus blocking viral entry. This approach has interesting potential applications for infection prophylaxis. In an effort to develop high affinity inhibitors of DC-SIGN mediated viral entry, we have synthesized a group of glycodendrimers of different valency that bear different carbohydrates or glycomimetic DC-SIGN ligands and have studied their DC-SIGN binding activity and antiviral properties both in an HIV and a Dengue infection model. Surface Plasmon Resonance (SPR) competition studies have demonstrated that the materials obtained bind efficiently to DC-SIGN with IC50s in the μm range, which depend on the nature of the ligand and on the valency of the scaffold. In particular, a hexavalent presentation of the DC-SIGN selective antagonist 4 displayed high potency, as well as improved accessibility and chemical stability relative to previously reported dendrimers. At low μm concentration the material was shown to block both DC-SIGN mediated uptake of DV by Raji cells and HIV trans-infection of T cells. es
dc.description.sponsorship European Union ITN Marie-Curie 213592 es
dc.format application/pdf es
dc.language.iso eng es
dc.publisher Elsevier es
dc.relation.ispartof Biomaterials, 35 (13), 4175-4184.
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 Internacional *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/ *
dc.subject Glycodendrimers es
dc.subject Glycomimetics es
dc.subject HIV es
dc.subject Dengue es
dc.subject DC-SIGN es
dc.subject Nanotechnology es
dc.title Identification of a promising multivalent inhibitor of the DC-SIGN dependent uptake of HIV-1 and Dengue virus es
dc.type info:eu-repo/semantics/article es
dc.type.version info:eu-repo/semantics/submittedVersion es
dc.rights.accessrights info:eu-repo/semantics/openAccess es
dc.relation.projectID 213592 es
dc.relation.publisherversion http://dx.doi.org/10.1016/j.biomaterials.2014.01.014 es
dc.identifier.doi 10.1016/j.biomaterials.2014.01.014 es
idus.format.extent 31 p. es
dc.journaltitle Biomaterials es
dc.publication.volumen 35 es
dc.publication.issue 13 es
dc.publication.initialPage 4175 es
dc.publication.endPage 4184 es
dc.contributor.funder European Union (UE)
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