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NTRC links built-in thioredoxin to light and sucrose in regulating starch synthesis in chloroplasts and amyloplasts

Opened Access NTRC links built-in thioredoxin to light and sucrose in regulating starch synthesis in chloroplasts and amyloplasts

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Autor: Michalska, Justyna
Zauber, Henrik
Buchanana, Bob B.
Cejudo Fernández, Francisco Javier
Geigenbergera, Peter
Departamento: Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular
Fecha: 2009
Publicado en: Proceedings of the National Academy of Sciences of the United States of America, 106 (24), 9908-9913.
Tipo de documento: Artículo
Resumen: Plants have an unusual plastid-localized NADP-thioredoxin reductase C (NTRC) containing both an NADP-thioredoxin reductase (NTR) and a thioredoxin (Trx) domain in a single polypeptide. Although NTRC is known to supply reductant for detoxifying hydrogen peroxide in the dark, its other functions are unknown. We now report that NTRC plays a previously unrecognized role in the redox regulation of ADP-glucose pyrophosphorylase (AGPase), a central enzyme of starch synthesis. When supplied NADPH, NTRC activated AGPase in vitro in a redox reaction that required the active site cysteines of both domains of the enzyme. In leaves, AGPase was activated in planta either by light or external feeding of sucrose in the dark. Leaves of an Arabidopsis NTRC KO mutant showed a decrease both in the extent of redox activation of AGPase and in the enhancement of starch synthesis either in the light (by 40-60%) or in the dark after treatment with external sucrose (by almost 100%). The light-dependent activat...
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Cita: Michalska, J., Zauber, H., Buchanana, B.B., Cejudo Fernández, F.J. y Geigenbergera, P. (2009). NTRC links built-in thioredoxin to light and sucrose in regulating starch synthesis in chloroplasts and amyloplasts. Proceedings of the National Academy of Sciences of the United States of America, 106 (24), 9908-9913.
Tamaño: 578.3Kb
Formato: PDF

URI: https://hdl.handle.net/11441/69422

DOI: 10.1073/pnas.0903559106

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