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dc.creatorRachwał, Kamilaes
dc.creatorLipa, Paulinaes
dc.creatorWojda, Iwonaes
dc.creatorVinardell González, José Maríaes
dc.creatorJanczarek, Monikaes
dc.date.accessioned2018-01-11T15:19:25Z
dc.date.available2018-01-11T15:19:25Z
dc.date.issued2017
dc.identifier.citationRachwał, K., Lipa, P., Wojda, I., Vinardell González, J.M. y Janczarek, M. (2017). Regulatory elements located in the upstream region of the Rhizobium leguminosarum rosR global regulator are essential for its transcription and mRNA stability. Genes, 8 (12), 388-.
dc.identifier.issn2073-4425es
dc.identifier.urihttp://hdl.handle.net/11441/68807
dc.description.abstractRhizobium leguminosarum bv. trifolii is a soil bacterium capable of establishing a symbiotic relationship with clover (Trifolium spp.). Previously, the rosR gene, encoding a global regulatory protein involved in motility, synthesis of cell-surface components, and other cellular processes was identified and characterized in this bacterium. This gene possesses a long upstream region that contains several regulatory motifs, including inverted repeats (IRs) of different lengths. So far, the role of these motifs in the regulation of rosR transcription has not been elucidated in detail. In this study, we performed a functional analysis of these motifs using a set of transcriptional rosR-lacZ fusions that contain mutations in these regions. The levels of rosR transcription for different mutant variants were evaluated in R. leguminosarum using both quantitative real-time PCR and β-galactosidase activity assays. Moreover, the stability of wild type rosR transcripts and those with mutations in the regulatory motifs was determined using an RNA decay assay and plasmids with mutations in different IRs located in the 5′ -untranslated region of the gene. The results show that transcription of rosR undergoes complex regulation, in which several regulatory elements located in the upstream region and some regulatory proteins are engaged. These include an upstream regulatory element, an extension of the -10 element containing three nucleotides TGn (TGn-extended -10 element), several IRs, and PraR repressor related to quorum sensing.es
dc.description.sponsorshipPolish National Science Centre DEC-2012/07/B/NZ1/00099es
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherMultidisciplinary Digital Publishing Institute (MDPI)es
dc.relation.ispartofGenes, 8 (12), 388-.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectGene expressiones
dc.subjectRhizobium leguminosarumes
dc.subjectRNA secondary structureses
dc.subjectRNA stabilityes
dc.subjectrosRes
dc.subjectSymbiotic bacteriaes
dc.subjectTranscription regulationes
dc.titleRegulatory elements located in the upstream region of the Rhizobium leguminosarum rosR global regulator are essential for its transcription and mRNA stabilityes
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessrightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Microbiologíaes
dc.relation.projectIDDEC-2012/07/B/NZ1/00099es
dc.relation.publisherversionhttp://dx.doi.org/10.3390/genes8120388es
dc.identifier.doi10.3390/genes8120388es
idus.format.extent21 p.es
dc.journaltitleGeneses
dc.publication.volumen8es
dc.publication.issue12es
dc.publication.initialPage388es
dc.contributor.funderNational Science Center. Poland

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