Repositorio de producción científica de la Universidad de Sevilla

Cytochrome c1 exhibits two binding sites for cytochrome c in plants

Opened Access Cytochrome c1 exhibits two binding sites for cytochrome c in plants

Citas

buscar en

Estadísticas
Icon
Exportar a
Autor: Moreno Beltrán, José Blas
Díaz Quintana, Antonio Jesús
González Arzola, Katiuska
Velázquez Campoy, Adrián
Rosa Acosta, Miguel Ángel de la
Díaz Moreno, Irene
Departamento: Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular
Fecha: 2014
Publicado en: Biochimica et Biophysica Acta - Bioenergetics, 1837, 1717-1729.
Tipo de documento: Artículo
Resumen: n plants, channeling of cytochrome c molecules between complexes III and IV has been purported to shuttle electrons within the supercomplexes instead of carrying electrons by random diffusion across the intermembrane bulk phase. However, the mode plant cytochrome c behaves inside a supercomplex such as the respirasome, formed by complexes I, III and IV, remains obscure from a structural point of view. Here, we report ab-initio Brownian dynamics calculations and nuclear magnetic resonance-driven docking computations showing two binding sites for plant cytochrome c at the head soluble domain of plant cytochrome c1, namely a non-productive (or distal) site with a long heme-to-heme distance and a functional (or proximal) site with the two heme groups close enough as to allow electron transfer. As inferred from isothermal titration calorimetry experiments, the two binding sites exhibit different equilibrium dissociation constants, for both reduced and oxidized species, that are all within ...
[Ver más]
Cita: Moreno Beltrán, J.B., Díaz Quintana, A.J., González Arzola, K., Velázquez Campoy, A., Rosa Acosta, M.Á.d.l. y Díaz Moreno, I. (2014). Cytochrome c1 exhibits two binding sites for cytochrome c in plants. Biochimica et Biophysica Acta - Bioenergetics, 1837, 1717-1729.
Tamaño: 1.086Mb
Formato: PDF

URI: http://hdl.handle.net/11441/68192

DOI: 10.1016/j.bbabio.2014.07.017

Ver versión del editor

Mostrar el registro completo del ítem


Esta obra está bajo una Licencia Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 Internacional

Este registro aparece en las siguientes colecciones