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CIELab chromaticity evolution to measure the binding free energy of non-colored biomolecules to gold nanoparticles

Opened Access CIELab chromaticity evolution to measure the binding free energy of non-colored biomolecules to gold nanoparticles

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Autor: Prado Gotor, Rafael
Jiménez Ruiz, Aila
Carnerero Panduro, José María
Grueso Molina, Elia María
Villa Bernáldez, Isaac
Departamento: Universidad de Sevilla. Departamento de Química Física
Fecha: 2015
Publicado en: RSC Advances, 5, 85039-85045.
Tipo de documento: Artículo
Resumen: We explore the possibilities of the CIELab chromaticity system parameters' evolution in order to study the interaction between two non-colored biological reactants, lysozyme and adenosine, and the red-colored, non-functionalized citrate gold nanoparticles. By measuring and quantifying the AuNP color changes, which are strongly dependant on their aggregation state, binding free energies are obtained for the first time for these two systems. Results are confirmed via an alternate fitting method which makes use of deconvolution parameters from experimental absorbance spectra. Binding free energies obtained through the use of both means are in good agreement with each other.
Cita: Prado Gotor, R., Jiménez Ruiz, A., Carnerero Panduro, J.M., Grueso Molina, E.M. y Villa Bernáldez, I. (2015). CIELab chromaticity evolution to measure the binding free energy of non-colored biomolecules to gold nanoparticles. RSC Advances, 5, 85039-85045.
Tamaño: 686.5Kb
Formato: PDF

URI: http://hdl.handle.net/11441/67958

DOI: 0.1039/c5ra15119h

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