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Uptake and intracellular activity of moxifloxacin in human neutrophils and tissue-cultured epithelial cells

 

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dc.creator Pascual Hernández, Álvaro es
dc.creator García Luque, Isabel es
dc.creator Ballesta Mudarra, Sofía es
dc.creator Perea Pérez, Evelio José es
dc.date.accessioned 2017-09-28T16:19:10Z
dc.date.available 2017-09-28T16:19:10Z
dc.date.issued 1999-02-15
dc.identifier.citation Pascual Hernández, Á., García Luque, I., Ballesta Mudarra, S. y Perea Pérez, E.J. (1999). Uptake and intracellular activity of moxifloxacin in human neutrophils and tissue-cultured epithelial cells. Antimicrobial Agents and Chemotherapy, 43 (1), 12-15.
dc.identifier.issn 0066-4804 (impreso) es
dc.identifier.issn 1098-6596 (electrónico) es
dc.identifier.uri http://hdl.handle.net/11441/64847
dc.description.abstract The penetration by moxifloxacin of human neutrophils (polymorphonuclear leukocytes [PMN]) and tissue-cultured epithelial cells (McCoy cells) was evaluated by a fluorometric assay. At extracellular concentrations of 5 mg/liter, the cellular-to-extracellular concentration ratios (C/E) of moxifloxacin in PMN and McCoy cells were 10.9 ± 1.0 and 8.7 ± 1.0, respectively (20 min; 37°C). The uptake of moxifloxacin by PMN was rapid, reversible, nonsaturable (at extracellular concentrations ranging from 1 to 50 μg/ml), and not affected by cell viability. The uptake of moxifloxacin was affected by external pH and the environmental temperature. The incubation of PMN in the presence of sodium fluoride, sodium cyanide, and carbonyl cyanide m-chlorophenylhydrazone significantly decreased the C/E of this agent. Neither PMN stimulation nor phagocytosis of opsonized Staphylococcus aureus significantly affected the uptake of moxifloxacin by human PMN. This agent, at concentrations of 0.5, 1, and 5 mg/liter, induced a significant reduction in the survival of intracellular S. aureus in human PMN. In summary, moxifloxacin reaches much higher intracellular concentrations within phagocytic and nonphagocytic cells than extracellular ones, remaining active inside the neutrophils. es
dc.format application/pdf es
dc.language.iso eng es
dc.publisher American Society for Microbiology es
dc.relation.ispartof Antimicrobial Agents and Chemotherapy, 43 (1), 12-15.
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 Internacional *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/ *
dc.title Uptake and intracellular activity of moxifloxacin in human neutrophils and tissue-cultured epithelial cells es
dc.type info:eu-repo/semantics/article es
dc.type.version info:eu-repo/semantics/publishedVersion es
dc.rights.accessrights info:eu-repo/semantics/openAccess es
dc.contributor.affiliation Universidad de Sevilla. Departamento de Microbiología es
idus.format.extent 4 p. es
dc.journaltitle Antimicrobial Agents and Chemotherapy es
dc.publication.volumen 43 es
dc.publication.issue 1 es
dc.publication.initialPage 12 es
dc.publication.endPage 15 es
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