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Roles of β-Lactamases and Porins in Activities of Carbapenems and Cephalosporins against Klebsiella pneumoniae

Opened Access Roles of β-Lactamases and Porins in Activities of Carbapenems and Cephalosporins against Klebsiella pneumoniae
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Autor: Martínez Martínez, Luis
Pascual Hernández, Álvaro
Hernández Allés, Santiago
Álvarez Díaz, Dolores
Suárez, Ana Isabel
Tran, John
Benedí, Vicente J.
Jacoby, George A.
Departamento: Universidad de Sevilla. Departamento de Microbiología
Fecha: 1999-07-01
Publicado en: Antimicrobial Agents and Chemotherapy, 43 (7), 1669-1673.
Tipo de documento: Artículo
Resumen: Two clinical isolates of extended-spectrum β-lactamase (ESBL)- producing Klebsiella pneumoniae were noted to be less susceptible than expected to imipenem. Both were missing outer membrane proteins that serve as channels for antibiotic entry. The role of β-lactamase in resistance was investigated by eliminating the original ESBL and introducing plasmids encoding various ESBLs and AmpC β-lactamase types, by studying the effect of an increased inoculum, and by evaluating interactions with β-lactamase inhibitors. The contribution of porin deficiency was investigated by restoring a functional ompK36 gene on a plasmid. Plasmids encoding AmpC-type β-lactamases provided resistance to imipenem (up to 64 μg/ml) and meropenem (up to 16 μg/ml) in strains deficient in porins. Carbapenem resistance showed little inoculum effect, was not affected by clavulanate but was blocked by BRL 42715, and was diminished if OmpK36 porin was restored. Plasmids encoding TEM- and SHV-type ESBLs conferred resistan...
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Cita: Martínez Martínez, L., Pascual Hernández, Á., Hernández Allés, S., Álvarez Díaz, D., Suárez, A.I., Tran, J.,...,Jacoby, G.A. (1999). Roles of β-Lactamases and Porins in Activities of Carbapenems and Cephalosporins against Klebsiella pneumoniae. Antimicrobial Agents and Chemotherapy, 43 (7), 1669-1673.
Tamaño: 77.69Kb
Formato: PDF

URI: http://hdl.handle.net/11441/64844

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