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In vivo selection of porin-deficient mutants of Klebsiella pneumoniae with increased resistance to cefoxitin and expanded-spectrum cephalosporins

Opened Access In vivo selection of porin-deficient mutants of Klebsiella pneumoniae with increased resistance to cefoxitin and expanded-spectrum cephalosporins
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Autor: Martínez Martínez, Luis
Hernández Allés, Santiago
Albertí, Sebastián
Tomás, Juan M.
Benedí, Vicente J.
Jacoby, George A.
Departamento: Universidad de Sevilla. Departamento de Microbiología
Fecha: 1996-02-01
Publicado en: Antimicrobial Agents and Chemotherapy, 40 (2), 342-348.
Tipo de documento: Artículo
Resumen: Four Klebsiella pneumoniae isolates (LB1, LB2, LB3, and LB4) with increased antimicrobial resistance were obtained from the same patient. The four isolates were indistinguishable in biotype, plasmid content, lipopolysaccharide, and DNA analysis by pulse-field gel electrophoresis. Isolate LB1 made TEM-1 and SHV-1 β-lactamases. Isolates LB2, LB3, and LB4 produced SHV-5 in addition to TEM-1 and SHV-1. MICs of cefoxitin, ceftazidime, and cefotaxime against LB1 were 4, 1, and 0.06 μg/ml, respectively. MICs of ceftazidime against K. pneumoniae LB2, LB3, and LB4 were >256 μg/ml, and those of cefotaxime were 2, 4, and 64 μg/ml, respectively. MICs of cefoxitin against K. pneumoniae LB2 and LB3 were 4 μg/ml, but that against K. pneumoniae LB4 was 128 μg/ml. K. pneumoniae LB4 could transfer resistance to ceftazidime and cefotaxime, but not that to cefoxitin, to Escherichia coli. Isolate LB4 and cefoxitin-resistant laboratory mutants lacked an outer membrane protein of about 35 kDa whose molecula...
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Cita: Martínez Martínez, L., Hernández Allés, S., Albertí, S., Tomás, J.M., Benedí, V.J. y Jacoby, G.A. (1996). In vivo selection of porin-deficient mutants of Klebsiella pneumoniae with increased resistance to cefoxitin and expanded-spectrum cephalosporins. Antimicrobial Agents and Chemotherapy, 40 (2), 342-348.
Tamaño: 473.1Kb
Formato: PDF

URI: http://hdl.handle.net/11441/64843

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