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The NADP+-isocitrate dehydrogenase gene (icd) is nitrogen regulated in cyanobacteria

Opened Access The NADP+-isocitrate dehydrogenase gene (icd) is nitrogen regulated in cyanobacteria
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Autor: Muro Pastor, Alicia María
Reyes Rosa, José Carlos
Florencio Bellido, Francisco Javier
Departamento: Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular
Fecha: 1996
Publicado en: Journal of Bacteriology, 178 (14), 4070-4076.
Tipo de documento: Artículo
Resumen: NADP+-isocitrate dehydrogenase (NADP+-IDH) activity and protein levels in crude extracts from the unicellular cyanobacterium Synechocystis sp. strain PCC 6803 and the filamentous, dinitrogen-fixing Anabaena sp. strain PCC 7120 were determined under different nitrogen conditions. The highest NADP+-IDH activity and protein accumulation were found under dinitrogen- fixing conditions for the Anabaena strain and under nitrogen starvation for Synechocystis sp. PCC 6803. The icd gene that encodes the NADP+-IDH from Synechocystis sp. strain PCC 6803 was cloned by heterologous hybridization with the previously isolated icd gene from Anabaena sp. strain PCC 7120. The two cyanobacterial icd genes show 81% sequence identity and share a typical 44-amino-acid region different from all the other icd genes sequenced so far. The icd gene seems to be essential for Synechocystis growth since attempts to generate a completely segregated icd mutant were unsuccessful. Transcripts of 2.0 and 1.6 kb were det...
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Cita: Muro Pastor, A.M., Reyes Rosa, J.C. y Florencio Bellido, F.J. (1996). The NADP+-isocitrate dehydrogenase gene (icd) is nitrogen regulated in cyanobacteria. Journal of Bacteriology, 178 (14), 4070-4076.
Tamaño: 477.9Kb
Formato: PDF

URI: http://hdl.handle.net/11441/64840

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