dc.creator | García Luque, Isabel | es |
dc.creator | Pascual Hernández, Álvaro | es |
dc.creator | Perea Pérez, Evelio José | es |
dc.date.accessioned | 2017-09-28T15:46:13Z | |
dc.date.available | 2017-09-28T15:46:13Z | |
dc.date.issued | 1994-01-01 | |
dc.identifier.citation | García Luque, I., Pascual Hernández, Á. y Perea Pérez, E.J. (1994). Intracellular penetration and activity of BAY Y 3118 in human polymorphonuclear leukocytes. Antimicrobial Agents and Chemotherapy, 38 (10), 2426-2429. | |
dc.identifier.issn | 0066-4804 (impreso) | es |
dc.identifier.issn | 1098-6596 (electrónico) | es |
dc.identifier.uri | http://hdl.handle.net/11441/64836 | |
dc.description.abstract | The penetration of a new quinolone (BAY Y 3118) into human polymorphonuclear leukocytes (PMNs) was evaluated by a fluorometric assay. The cellular concentration-to-extracellular concentration (C/E) ratio was higher than 6.3 at extracellular concentrations ranging from 2 to 100 mg/liter. The uptake of BAY Y 3118 was rapid, reversible and nonsaturable. The intracellular penetration of BAY Y 3118 was significantly affected by environmental temperature (C/E ratio at 4°C, 5.4 ± 0.5; control, 7.5 ± 0.9; P < 0.05) and cell viability (C/E ratio in dead PMNs, 5.5 ± 0.8; control, 7.5 ± 0.9; P < 0.05), but it was not affected by metabolic inhibitors. The ingestion of opsonized zymosan or opsonized Staphylococcus aureus significantly decreased the levels of PMN-associated BAY Y 3118. Cell stimulation by a membrane activator, however, significantly increased the intracellular concentration of this quinolone. At therapeutic extracellular concentrations (0.5, 2, and 5 mg/liter), BAY Y 3118 showed intracellular activity greater than that of ciprofloxacin against S. aureus in human PMNs. It was concluded that BAY Y 3118 reaches high intracellular concentrations within human PMNs and remains active intracellularly. | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | American Society for Microbiology | es |
dc.relation.ispartof | Antimicrobial Agents and Chemotherapy, 38 (10), 2426-2429. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Intracellular penetration and activity of BAY Y 3118 in human polymorphonuclear leukocytes | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Microbiología | es |
idus.format.extent | 4 p. | es |
dc.journaltitle | Antimicrobial Agents and Chemotherapy | es |
dc.publication.volumen | 38 | es |
dc.publication.issue | 10 | es |
dc.publication.initialPage | 2426 | es |
dc.publication.endPage | 2429 | es |