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Development of a new fluorescent reporter:operator system: location of AraC regulated genes in Escherichia coli K-12

 

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dc.creator Sellars, Laura E. es
dc.creator Bryant, Jack A. es
dc.creator Sánchez Romero, María Antonia es
dc.creator Sánchez Morán, Eugenio es
dc.creator Busby, Stephen J. W. es
dc.creator Lee, David J. es
dc.date.accessioned 2017-09-20T17:31:39Z
dc.date.available 2017-09-20T17:31:39Z
dc.date.issued 2017
dc.identifier.citation Sellars, L.E., Bryant, J.A., Sánchez Romero, M.A., Sánchez Morán, E., Busby, S.J.W. y Lee, D.J. (2017). Development of a new fluorescent reporter:operator system: location of AraC regulated genes in Escherichia coli K-12. BMC Microbiology, 17 (170), 1-10.
dc.identifier.issn 1471-2180 es
dc.identifier.uri http://hdl.handle.net/11441/64534
dc.description.abstract In bacteria, many transcription activator and repressor proteins regulate multiple transcription units that are often distally distributed on the bacterial genome. To investigate the subcellular location of DNA bound proteins in the folded bacterial nucleoid, fluorescent reporters have been developed which can be targeted to specific DNA operator sites. Such Fluorescent Reporter-Operator System (FROS) probes consist of a fluorescent protein fused to a DNA binding protein, which binds to an array of DNA operator sites located within the genome. Here we have developed a new FROS probe using the Escherichia coli MalI transcription factor, fused to mCherry fluorescent protein. We have used this in combination with a LacI repressor::GFP protein based FROS probe to assess the cellular location of commonly regulated transcription units that are distal on the Escherichia coli genome. es
dc.format application/pdf es
dc.language.iso eng es
dc.publisher BioMed Central es
dc.relation.ispartof BMC Microbiology, 17 (170), 1-10.
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 Internacional *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/ *
dc.subject FROS es
dc.subject GFP es
dc.subject Fluorescent microscopy es
dc.subject Nucleoid es
dc.subject Escherichia coli es
dc.title Development of a new fluorescent reporter:operator system: location of AraC regulated genes in Escherichia coli K-12 es
dc.type info:eu-repo/semantics/article es
dc.type.version info:eu-repo/semantics/publishedVersion es
dc.rights.accessrights info:eu-repo/semantics/openAccess es
dc.contributor.affiliation Universidad de Sevilla. Departamento de Genética es
dc.relation.publisherversion 10.1186/s12866-017-1079-2 es
dc.identifier.doi http://dx.doi.org/10.1186/s12866-017-1079-2 es
idus.format.extent 11 p. es
dc.journaltitle BMC Microbiology es
dc.publication.volumen 17 es
dc.publication.issue 170 es
dc.publication.initialPage 1 es
dc.publication.endPage 10 es
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