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Development of a new fluorescent reporter:operator system: location of AraC regulated genes in Escherichia coli K-12

 

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Opened Access Development of a new fluorescent reporter:operator system: location of AraC regulated genes in Escherichia coli K-12
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Author: Sellars, Laura E.
Bryant, Jack A.
Sánchez Romero, María Antonia
Sánchez Morán, Eugenio
Busby, Stephen J. W.
Lee, David J.
Department: Universidad de Sevilla. Departamento de Genética
Date: 2017
Published in: BMC Microbiology, 17 (170), 1-10.
Document type: Article
Abstract: In bacteria, many transcription activator and repressor proteins regulate multiple transcription units that are often distally distributed on the bacterial genome. To investigate the subcellular location of DNA bound proteins in the folded bacterial nucleoid, fluorescent reporters have been developed which can be targeted to specific DNA operator sites. Such Fluorescent Reporter-Operator System (FROS) probes consist of a fluorescent protein fused to a DNA binding protein, which binds to an array of DNA operator sites located within the genome. Here we have developed a new FROS probe using the Escherichia coli MalI transcription factor, fused to mCherry fluorescent protein. We have used this in combination with a LacI repressor::GFP protein based FROS probe to assess the cellular location of commonly regulated transcription units that are distal on the Escherichia coli genome.
Cite: Sellars, L.E., Bryant, J.A., Sánchez Romero, M.A., Sánchez Morán, E., Busby, S.J.W. y Lee, D.J. (2017). Development of a new fluorescent reporter:operator system: location of AraC regulated genes in Escherichia coli K-12. BMC Microbiology, 17 (170), 1-10.
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URI: http://hdl.handle.net/11441/64534

DOI: http://dx.doi.org/10.1186/s12866-017-1079-2

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