Repositorio de producción científica de la Universidad de Sevilla

TDP2/TTRAP Is the Major 5′-Tyrosyl DNA Phosphodiesterase Activity in Vertebrate Cells and Is Critical for Cellular Resistance to Topoisomerase II-induced DNA Damage

Opened Access TDP2/TTRAP Is the Major 5′-Tyrosyl DNA Phosphodiesterase Activity in Vertebrate Cells and Is Critical for Cellular Resistance to Topoisomerase II-induced DNA Damage

Citas

buscar en

Estadísticas
Icon
Exportar a
Autor: Zeng, Zhihong
Cortés Ledesma, Felipe
El Khamisy, Sherif F.
Caldecott, Keith W.
Fecha: 2011
Publicado en: Journal of Biological Chemistry, 286 (1), 403-409.
Tipo de documento: Artículo
Resumen: Topoisomerase II (Top2) activity involves an intermediate in which the topoisomerase is covalently bound to a DNA double-strand break via a 5′-phosphotyrosyl bond. Although these intermediates are normally transient, they can be stabilized by antitumor agents that act as Top2 “poisons,” resulting in the induction of cytotoxic double-strand breaks, and they are implicated in the formation of site-specific translocations that are commonly associated with cancer. Recently, we revealed that TRAF and TNF receptor-associated protein (TTRAP) is a 5′-tyrosyl DNA phosphodiesterase (5′-TDP) that can cleave 5′-phosphotyrosyl bonds, and we denoted this protein tyrosyl DNA phosphodiesterase-2 (TDP2). Here, we have generated TDP2-deleted DT40 cells, and we show that TDP2 is the major if not the only 5′-TDP activity present in vertebrate cells. We also show that TDP2-deleted DT40 cells are highly sensitive to the anticancer Top2 poison, etoposide, but are not hypersensitive to the Top1 poison campto...
[Ver más]
Cita: Zeng, Z., Cortés Ledesma, F., El Khamisy, S.F. y Caldecott, .W. (2011). TDP2/TTRAP Is the Major 5′-Tyrosyl DNA Phosphodiesterase Activity in Vertebrate Cells and Is Critical for Cellular Resistance to Topoisomerase II-induced DNA Damage. Journal of Biological Chemistry, 286 (1), 403-409.
Tamaño: 2.088Mb
Formato: PDF

URI: http://hdl.handle.net/11441/64488

DOI: 10.1074/jbc.M110.181016

Ver versión del editor

Mostrar el registro completo del ítem


Esta obra está bajo una Licencia Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 Internacional

Este registro aparece en las siguientes colecciones