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Immunohistochemical assessment of Pax8 expression during pancreatic islet development and in human neuroendocrine tumors

Opened Access Immunohistochemical assessment of Pax8 expression during pancreatic islet development and in human neuroendocrine tumors

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Autor: Lorenzo, Petra I.
Jiménez Moreno, Carmen María
Delgado, Irene
Cobo Vuilleumier, Nadia
Meier, Rafael
Gómez Izquierdo, Lourdes
Berney, Thierry
García Carbonero, Rocío
Rojas, Anabel
Gauthier, Benoit
Fecha: 2011
Publicado en: Histochemistry and Cell Biology, 136 (5), 595-607.
Tipo de documento: Artículo
Resumen: The paired box transcription factor Pax8 is critical for development of the eye, thyroid gland as well as the urinary and reproductive organs. In adult, Pax8 overexpression is associated with kidney, ovarian and thyroid tumors and has emerged as a specific marker for these cancers. Recently, Pax8 expression was also reported in human pancreatic islets and in neuroendocrine tumors, identifying Pax8 as a novel member of the Pax family expressed in the pancreas. Herein, we sought to provide a comprehensive analysis of Pax8 expression during pancreogenesis and in adult islets. Immunohistochemical analysis using the most employed Pax8 polyclonal antibody revealed strong nuclear staining in the developing mouse pancreas and in mature human and mouse islets. Astonishingly, Pax8 mRNA in mouse islets was undetectable while human islets exhibited low levels. These discrepancies raised the possibility of antibody cross-reactivity. This premise was confirmed by demonstrating that the polyclonal P...
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Cita: García Carbonero, R., Lorenzo, P.I., Jiménez Moreno, C.M., Delgado, I., Gómez Izquierdo, L. y Rojas, A. (2011). Immunohistochemical assessment of Pax8 expression during pancreatic islet development and in human neuroendocrine tumors. Histochemistry and Cell Biology, 136 (5), 595-607.
Tamaño: 2.523Mb
Formato: PDF

URI: http://hdl.handle.net/11441/64455

DOI: 10.1007/s00418-011-0866-3

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