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miR-125a, miR-139 and miR-324 contribute to Urocortin protection against myocardial ischemia-reperfusion injury

Opened Access miR-125a, miR-139 and miR-324 contribute to Urocortin protection against myocardial ischemia-reperfusion injury

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Autor: Díaz Carrasco, Ignacio
Calderón Sánchez, Eva María
Toro, Raquel del
Ávila Médina, Javier
Sánchez de Rojas de Pedro ,Eva
Domínguez Rodríguez, Alejandro
Rosado, Juan Antonio
Hmadcha, Abdelkrim
Fecha: 2017
Tipo de documento: Artículo
Resumen: Urocortin 1 and 2 (Ucn-1 and Ucn-2) have established protective actions against myocardial ischemia-reperfusion (I/R) injuries. However, little is known about their role in posttranscriptional regulation in the process of cardioprotection. Herein, we investigated whether microRNAs play a role in urocortin-induced cardioprotection. Administration of Ucn-1 and Ucn-2 at the beginning of reperfusion significantly restored cardiac function, as evidenced ex vivo in Langendorff-perfused rat hearts and in vivo in rat subjected to I/R. Experiments using microarray and qRT-PCR determined that the addition of Ucn-1 at reperfusion modulated the expression of several miRNAs with unknown role in cardiac protection. Ucn-1 enhanced the expression of miR-125a-3p, miR-324-3p; meanwhile it decreased miR-139-3p. Similarly, intravenous infusion of Ucn-2 in rat model of I/R mimicked the effect of Ucn-1 on miR-324-3p and miR-139-3p. The effect of Ucn-1 involves the activation of corticotropin-releasing fact...
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Tamaño: 2.482Mb
Formato: PDF

URI: http://hdl.handle.net/11441/64425

DOI: /10.1038/s41598-017-09198-x

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