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Anti-inflammatory activity of lupine (Lupinus angustifolius L.) protein hydrolysates in THP-1-derived macrophages

Opened Access Anti-inflammatory activity of lupine (Lupinus angustifolius L.) protein hydrolysates in THP-1-derived macrophages

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Autor: Millán Linares, María del Carmen
Bermúdez, Beatriz
Yust Escobar, María del Mar
Millán, Francisco
Pedroche, Justo
Departamento: Universidad de Sevilla. Departamento de Ingeniería Química
Fecha: 2014
Publicado en: Journal of Functional Foods, 8, 224-233.
Tipo de documento: Artículo
Resumen: The effect of two different lupine protein hydrolysates (LPHs) on in vitro macrophage activation in a THP-1-derived macrophage model was investigated. THP-1-derived macrophages were exposed to RPMI medium containing two LPHs obtained by enzymatic hydrolysis using two different proteases: Izyme AL and Alcalase 2.4 L. Cytokine's expression was measured by quantitative PCR. THP-1-derived macrophages exhibited attenuated expression of proinflammatory cytokines (tumor necrosis factor (TNF), IL-6, IL-1β) and increased expression of anti-inflammatory marker genes (chemokine (C-C motif) ligand 18 (CCL18)) relative to control without LPH. The anti-inflammatory effect of both hydrolysates favored M2 polarization by quenching C-C chemokine receptor type 2 (CCR2) expression and migratory capacity. Furthermore, LPHs significantly decreased nitric oxide production. Moreover, LPHs promoted the survival of human THP-1-derived macrophages. Therefore, inclusion of LPHs in foods may help to prevent chro...
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Cita: Millán Linares, M.d.C., Bermúdez, B., Yust Escobar, M.d.M., Millán, F. y Pedroche, J. (2014). Anti-inflammatory activity of lupine (Lupinus angustifolius L.) protein hydrolysates in THP-1-derived macrophages. Journal of Functional Foods, 8, 224-233.
Tamaño: 443.6Kb
Formato: PDF

URI: http://hdl.handle.net/11441/64412

DOI: 10.1016/j.jff.2014.03.020

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