Repositorio de producción científica de la Universidad de Sevilla

EGF-induced adipose tissue mesothelial cells undergo functional vascular smooth muscle differentiation

Opened Access EGF-induced adipose tissue mesothelial cells undergo functional vascular smooth muscle differentiation

Citas

buscar en

Estadísticas
Icon
Exportar a
Autor: Lachaud, C. C.
López Beas, Javier
Soria, B.
Hmadcha, Abdelkrim
Fecha: 2014
Publicado en: Cell Death and Disease, 5 (6), e1304-1-e1304-11.
Tipo de documento: Artículo
Resumen: Recent studies suggested that the post-natal mesothelium retain differentiative potential of the embryonic mesothelium, which generates fibroblasts and vascular smooth muscle cells (VSMCs), in developing coelomic organs via epithelial-to-mesenchymal transition (EMT). Whether adult mesothelial cells (MCs) are able to give rise to functional VSMCs in vitro and which are the factors and mechanisms directing this process remain largely unknown. Here, we isolated adipose tissue MCs (ATMCs) from adult mice, and demonstrated that ATMCs cultured in a serum-containing media supplemented with epidermal growth factor (EGF) efficiently increased both their proliferation and EMT above levels found in only serum-containing media cultures. EGF-induced ATMCs gained phosphorylation of the EGF receptor and activated simultaneously ILK/Erk1/2, PI3K/Akt and Smad2/3-dependent pathways. Sequential subculture onto collagen-I surface efficiently improved their vasculogenic EMT towards cells featuring VSMCs (...
[Ver más]
Cita: Lachaud, C.C., López Beas, J., Soria, B. y Hmadcha, A. (2014). EGF-induced adipose tissue mesothelial cells undergo functional vascular smooth muscle differentiation. Cell Death and Disease, 5 (6), e1304-1-e1304-11.
Tamaño: 3.726Mb
Formato: PDF

URI: http://hdl.handle.net/11441/64403

DOI: 10.1038/cddis.2014.271

Ver versión del editor

Mostrar el registro completo del ítem


Esta obra está bajo una Licencia Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 Internacional

Este registro aparece en las siguientes colecciones