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OxyR-dependent formation of DNA methylation patterns in OpvABOFF and OpvABON cell lineages of Salmonella enterica

Opened Access OxyR-dependent formation of DNA methylation patterns in OpvABOFF and OpvABON cell lineages of Salmonella enterica

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Autor: Cota García, Ignacio
Bunk, Boyke
Sproer, Cathryn
Overmann, Joerg
Koenig, Christoph
Casadesús Pursals, Josep
Departamento: Universidad de Sevilla. Departamento de Genética
Fecha: 2015
Publicado en: Nucleic Acids Research, 44 (8), 3595-3609.
Tipo de documento: Artículo
Resumen: Phase variation of the Salmonella enterica opvAB operon generates a bacterial lineage with standard lipopolysaccharide structure (OpvABOFF) and a lineage with shorter O-antigen chains (OpvABON). Regulation of OpvAB lineage formation is transcriptional, and is controlled by the LysR-type factor OxyR and by DNA adenine methylation. The opvAB regulatory region contains four sites for OxyR binding (OBSA-D), and four methylatable GATC motifs (GATC1-4). OpvABOFF and OpvABON cell lineages display opposite DNA methylation patterns in the opvAB regulatory region: (i) in the OpvABOFF state, GATC1 and GATC3 are non-methylated, whereas GATC2 and GATC4 are methylated; (ii) in the OpvABON state, GATC2 and GATC4 are non-methylated, whereas GATC1 and GATC3 are methylated. We provide evidence that such DNA methylation patterns are generated by OxyR binding. The higher stability of the OpvABOFF lineage may be caused by binding of OxyR to sites that are identical to the consensus (OBSA and OBSc), while ...
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Cita: Cota García, I., Bunk, B., Sproer, C., Overmann, J., Koenig, C. y Casadesús Pursals, J. (2015). OxyR-dependent formation of DNA methylation patterns in OpvABOFF and OpvABON cell lineages of Salmonella enterica. Nucleic Acids Research, 44 (8), 3595-3609.
Tamaño: 8.033Mb
Formato: PDF

URI: http://hdl.handle.net/11441/64107

DOI: 10.1093/nar/gkv1483

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