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Functional analysis of the pathways for 2-Cys peroxiredoxin reduction in Arabidopsis thaliana chloroplasts

Opened Access Functional analysis of the pathways for 2-Cys peroxiredoxin reduction in Arabidopsis thaliana chloroplasts

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Autor: Pulido, Pablo
Espínola, María Cristina
Kirchsteiger, Kerstin
Guinea, Manuel
Cejudo Fernández, Francisco Javier
González García, María de la Cruz
Departamento: Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular
Fecha: 2010
Publicado en: Journal of Experimental Botany
Tipo de documento: Artículo
Resumen: Photosynthesis is a process that inevitably produces reactive oxygen species, such as hydrogen peroxide, which is reduced by chloroplast-localized detoxification mechanisms one of which involves 2-Cys peroxiredoxins (2-Cys Prxs). Arabidopsis chloroplasts contain two very similar 2-Cys Prxs (denoted A and B). These enzymes are reduced by two pathways: NADPH thioredoxin reductase C (NTRC), which uses NADPH as source of reducing power; and plastidial thioredoxins (Trxs) coupled to photosynthetically reduced ferredoxin of which Trx x is the most efficient reductant in vitro. With the aim of establishing the functional relationship between NTRC, Trx x, and 2-Cys Prxs in vivo, an Arabidopsis Trx x knock-out mutant has been identified and a double mutant (denoted Δ2cp) with <5% of 2-Cys Prx content has been generated. The phenotypes of the three mutants, ntrc, trxx, and Δ2cp, were compared under standard growth conditions and in response to continuous light or prolonged darkness and oxidativ...
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Cita: Pulido, P., Espínola, M.C., Kirchsteiger, K., Guinea, M., Cejudo Fernández, F.J. y González García, M.d.l.C. (2010). Functional analysis of the pathways for 2-Cys peroxiredoxin reduction in Arabidopsis thaliana chloroplasts. Journal of Experimental Botany
Tamaño: 578.2Kb
Formato: PDF

URI: http://hdl.handle.net/11441/64074

DOI: 10.1093/jxb/erq218

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