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Generation of a Vero-Based Packaging Cell Line to Produce SV40 Gene Delivery Vectors for Use in Clinical Gene Therapy Studies

Opened Access Generation of a Vero-Based Packaging Cell Line to Produce SV40 Gene Delivery Vectors for Use in Clinical Gene Therapy Studies

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Autor: Toscano, Miguel G.
Velden, Jeroen van der
Werf, Sybrand van der
Odijk, Machteld
Camacho García, Rafael J.
Herrera Gómez, Irene G.
Departamento: Universidad de Sevilla. Departamento de Fisiología Médica y Biofísica
Fecha: 2017
Publicado en: Molecular Therapy - Methods and Clinical Development, 6, 124-134.
Tipo de documento: Artículo
Resumen: Replication-defective (RD) recombinant simian virus 40 (SV40)-based gene delivery vectors hold a great potential for clinical applications because of their presumed non-immunogenicity and capacity to induce immune tolerance to the transgene products in humans. However, the clinical use of SV40 vectors has been hampered by the lack of a packaging cell line that produces replication-competent (RC) free SV40 particles in the vector production process. To solve this problem, we have adapted the current SV40 vector genome used for the production of vector particles and generated a novel Vero-based packaging cell line named SuperVero that exclusively expresses the SV40 large T antigen. SuperVero cells produce similar numbers of SV40 vector particles compared to the currently used packaging cell lines, albeit in the absence of contaminating RC SV40 particles. Our unique SV40 vector platform named SVac paves the way to clinically test a whole new generation of SV40-based therapeutics for a br...
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Cita: Toscano, M.G., Velden, J.v.d., Werf, S.v.d., Odijk, M., Camacho García, R.J. y Herrera Gómez, I.G. (2017). Generation of a Vero-Based Packaging Cell Line to Produce SV40 Gene Delivery Vectors for Use in Clinical Gene Therapy Studies. Molecular Therapy - Methods and Clinical Development, 6, 124-134.
Tamaño: 1.432Mb
Formato: PDF

URI: http://hdl.handle.net/11441/63908

DOI: 10.1016/j.omtm.2017.06.007

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