Repositorio de producción científica de la Universidad de Sevilla

Regulation of glutamine synthetase activity in the unicellular cyanobacterium Synechocystis sp. strain PCC 6803 by the nitrogen source: Effect of ammonium

Opened Access Regulation of glutamine synthetase activity in the unicellular cyanobacterium Synechocystis sp. strain PCC 6803 by the nitrogen source: Effect of ammonium
Estadísticas
Icon
Exportar a
Autor: Mérida Berlanga, Ángel
Candau Chacón, Pedro
Florencio Bellido, Francisco Javier
Departamento: Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular
Fecha: 1991
Publicado en: Journal of Bacteriology, 173 (13), 4095-4100.
Tipo de documento: Artículo
Resumen: Glutamine synthetase activity from Synechocystis sp. strain PCC 6803 is regulated as a function of the nitrogen source available in the medium. Addition of 0.25 mM NH4Cl to nitrate-grown cells promotes a clear short-term inactivation of glutamine synthetase, whose enzyme activity decreases to 5 to 10% of the initial value in 25 min. The intracellular levels of glutamine, determined under various conditions, taken together with the results obtained with azaserine (an inhibitor of transamidases), rule out the possibility that glutamine per se is responsible for glutamine synthetase inactivation. Nitrogen starvation attenuates the ammonium-mediated glutamine synthetase inactivation, indicating that glutamine synthetase regulation is modulated through the internal balance between carbon-nitrogen compounds and carbon compounds. The parallelism observed between the glutamine synthetase activity and the internal concentration of α-ketoglutarate suggests that this metabolite could play a role...
[Ver más]
Cita: Mérida Berlanga, Á., Candau Chacón, P. y Florencio Bellido, F.J. (1991). Regulation of glutamine synthetase activity in the unicellular cyanobacterium Synechocystis sp. strain PCC 6803 by the nitrogen source: Effect of ammonium. Journal of Bacteriology, 173 (13), 4095-4100.
Tamaño: 1.332Mb
Formato: PDF

URI: http://hdl.handle.net/11441/63351

Mostrar el registro completo del ítem


Esta obra está bajo una Licencia Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 Internacional

Este registro aparece en las siguientes colecciones