Repositorio de producción científica de la Universidad de Sevilla

Direct Detection of Rifampin- and Isoniazid-Resistant Mycobacterium tuberculosis in Auramine-Rhodamine-Positive Sputum Specimens by Real-Time PCR

Opened Access Direct Detection of Rifampin- and Isoniazid-Resistant Mycobacterium tuberculosis in Auramine-Rhodamine-Positive Sputum Specimens by Real-Time PCR

Citas

buscar en

Estadísticas
Icon
Exportar a
Autor: Ruiz Pérez, Maite
Torres Sánchez, María José
Llanos Rodríguez, Ana Cristina
Arroyos, Aurelio
Palomares Folía, José Carlos
Aznar Martín, Javier
Departamento: Universidad de Sevilla. Departamento de Microbiología
Fecha: 2004
Publicado en: Journal of Clinical Microbiology, 42 (4), 1585-1589.
Tipo de documento: Artículo
Resumen: Our objective was to evaluate the feasibility of a molecular assay based on a real-time PCR technique, carried out with a LightCycler instrument (Roche Biochemicals), to identify Mycobacterium tuberculosis bacilli and to detect rifampin and isoniazid resistance in DNA extracts from sputum samples. We studied three genes: rpoB, which is associated with rifampin resistance, and katG and inhA, which are associated with isoniazid resistance. A total of 205 sputum samples collected from 108 patients diagnosed with pulmonary tuberculosis with positive auramine-rhodamine-staining (AR) sputum samples, were tested. The sensitivities of the LightCycler PCR assay for the positive AR specimens was 97.5% (200 of 205) for rpoB and inhA genes and 96.5% (198 of 205) for the katG gene. For the total number of patients tested, the sensitivity was 100% (108 of 108 patients) for rifampin, whereas the sensitivity was 98.1% (106 of 108 patients) for isoniazid. Full agreement was found with the Bactec MGIT ...
[Ver más]
Cita: Ruíz Pérez, M., Torres Sánchez, M.J., Llanos Rodríguez, A.C., Arroyos, A., Palomares Folia, J.C. y Aznar Martín, J. (2004). Direct Detection of Rifampin- and Isoniazid-Resistant Mycobacterium tuberculosis in Auramine-Rhodamine-Positive Sputum Specimens by Real-Time PCR. Journal of Clinical Microbiology, 42 (4), 1585-1589.
Tamaño: 66.32Kb
Formato: PDF

URI: http://hdl.handle.net/11441/63332

DOI: 10.1128/JCM.42.4.1585-1589.2004

Ver versión del editor

Mostrar el registro completo del ítem


Esta obra está bajo una Licencia Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 Internacional

Este registro aparece en las siguientes colecciones