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The global nitrogen regulator NtcA regulates transcription of the signal transducer P(II) (GlnB) and influences its phosphorylation level in response to nitrogen and carbon supplies in the cyanobacterium Synechococcus sp. strain PCC 7942

Opened Access The global nitrogen regulator NtcA regulates transcription of the signal transducer P(II) (GlnB) and influences its phosphorylation level in response to nitrogen and carbon supplies in the cyanobacterium Synechococcus sp. strain PCC 7942
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Autor: Lee, Hyun-Mi
Vázquez Bermúdez, María Félix
Tandeau De Marsac, Nicole
Departamento: Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular
Fecha: 1999
Publicado en: Journal of Bacteriology, 181 (9), 2697-2702.
Tipo de documento: Artículo
Resumen: The P(II) protein is encoded by a unique glnB gene in Synechococcus sp. strain PCC 7942. Its expression has been analyzed in the wild type and in NtcA-null mutant cells grown under different conditions of nitrogen and carbon supply. RNA-DNA hybridization experiments revealed the presence of one transcript species 680 nucleotides long, whatever the nutrient conditions tested. A second transcript species, 620 nucleotides long, absent in the NtcA null mutant, was observed in wild-type cells that were nitrogen starved for 2 h under both high and low CO2 and in the presence of nitrate under a high CO2 concentration. Primer extension analysis indicated that the two transcript species are generated from two tandem promoters, a σ70 Escherichia coli-type promoter and an NtcA-dependent promoter, located 120 and 53 nucleotides, respectively, from the glnB initiation codon. The NtcA- dependent promoter is up-regulated under the conditions mentioned above, while the σ70 E. coli-type promoter displ...
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Cita: Lee, H., Vázquez Bermúdez, M.F. y Tandeau De Marsac, N. (199). The global nitrogen regulator NtcA regulates transcription of the signal transducer P(II) (GlnB) and influences its phosphorylation level in response to nitrogen and carbon supplies in the cyanobacterium Synechococcus sp. strain PCC 7942. Journal of Bacteriology, 181 (9), 2697-2702.
Tamaño: 510.9Kb
Formato: PDF

URI: http://hdl.handle.net/11441/63114

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