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Light-dependent regulation of cyanobacterial phytochrome expression


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dc.creator García Domínguez, Mario es
dc.creator Muro Pastor, Alicia María es
dc.creator Reyes Rosa, José Carlos es
dc.creator Florencio Bellido, Francisco Javier es 2017-07-24T10:56:52Z 2017-07-24T10:56:52Z 2000-01
dc.identifier.citation García Domínguez, M., Muro Pastor, A.M., Reyes Rosa, J.C. y Florencio Bellido, F.J. (2000). Light-dependent regulation of cyanobacterial phytochrome expression. Journal of Bacteriology, 182 (1), 38-44.
dc.identifier.issn 0021-9193 (impreso) es
dc.identifier.issn 1098-5530 (electronico) es
dc.description.abstract A histidine kinase protein (Cph1) with sequence homology and spectral characteristics very similar to those of the plant phytochrome has been recently identified in the cyanobacterium Synechocystis sp. strain PCC 6803. Cph1 together with Rcp1 (a protein homologue to the response regulator CheY) forms a light-regulated two-component system whose function is presently unknown. Levels of cph1 rcp1 mRNA increase in the dark and decrease upon reillumination. A dark-mediated increase in cph1 rcp1 mRNA levels was inhibited by the presence of glucose, but not by inhibition of the photosynthetic electron flow. The half-life of cph1 rcp1 transcript in the light was about fourfold shorter than in the dark, indicating that control of cph1 rcp1 transcript stability is one of the mechanisms by which light regulates expression of the cyanobacterial phytochrome. After 15 min of darkness, 3-min pulses of red, blue, green, and far-red light were equally efficient in decreasing the cph1 rcp1 mRNA levels. Red light downregulation was not reversed by far-red light, suggesting that cph1 rcp1 mRNA levels are not controlled by a phytochrome-like photoreceptor. Furthermore, a Synechocystis strain containing an H538R Cph1 point mutation, unable to phosphorylate Rcp1, shows normal light-dark regulation of the cph1 rcp1 transcript levels. Our data suggest a role of cyanobacterial phytochrome in the control of processes required for adaptation in light-dark and dark-light transitions. es
dc.description.sponsorship DGESID PB97-0732 es
dc.description.sponsorship Junta de Andalucía CV1-0112 es
dc.format application/pdf es
dc.language.iso eng es
dc.publisher American Society for Microbiology es
dc.relation.ispartof Journal of Bacteriology, 182 (1), 38-44.
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 Internacional *
dc.rights.uri *
dc.title Light-dependent regulation of cyanobacterial phytochrome expression es
dc.type info:eu-repo/semantics/article es
dc.type.version info:eu-repo/semantics/publishedVersion es
dc.rights.accessrights info:eu-repo/semantics/openAccess es
dc.contributor.affiliation Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular es
dc.relation.projectID PB97-0732 es
dc.relation.projectID CV1-0112 es
idus.format.extent 7 p. es
dc.journaltitle Journal of Bacteriology es
dc.publication.volumen 182 es
dc.publication.issue 1 es
dc.publication.initialPage 38 es
dc.publication.endPage 44 es
dc.contributor.funder Junta de Andalucía
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