dc.creator | Camacho Fernández, Eva María | es |
dc.creator | Serna, Ana | es |
dc.creator | Madrid, Cristina | es |
dc.creator | Marqués Martín, Silvia | es |
dc.creator | Fernández, Raúl | es |
dc.creator | Cruz, Fernando de la | es |
dc.creator | Juárez, Antonio | es |
dc.creator | Casadesús Pursals, Josep | es |
dc.date.accessioned | 2017-07-24T08:32:16Z | |
dc.date.available | 2017-07-24T08:32:16Z | |
dc.date.issued | 2005-08 | |
dc.identifier.citation | Camacho Fernández, E.M., Serna, A., Madrid, C., Marqués Martín, S., Fernández, R., de la Cruz, F.,...,Casadesús Pursals, J. (2005). Regulation of finP transcription by DNA adenine methylation in the virulence plasmid of Salmonella enterica. Journal of Bacteriology, 187 (16), 5691-5699. | |
dc.identifier.issn | 0021-9193 (impreso) | es |
dc.identifier.issn | 1098-5530 (electronico) | es |
dc.identifier.uri | http://hdl.handle.net/11441/62931 | |
dc.description.abstract | DNA adenine methylase (Dam -) mutants of Salmonella enterica serovar Typhimurium contain reduced levels of FinP RNA encoded on the virulence plasmid. Dam methylation appears to regulate finP transcription, rather than FinP RNA stability or turnover. The finP promoter includes canonical -10 and -35 modules and depends on the σ 70 factor. Regulation of finP transcription by Dam methylation does not require DNA sequences upstream from the -35 module, indicating that Dam acts at the promoter itself or downstream. Unexpectedly, a GATC site overlapping with the -10 module is likewise dispensable for Dam-mediated regulation. These observations indicate that Dam methylation regulates finP transcription indirectly and suggest the involvement of a host factor(s) responsive to the Dam methylation state of the cell. We provide evidence that one such factor is the nucleoid protein H-NS, which acts as a repressor of finP transcription in a Dam - background. H-NS also restrains transcription of the overlapping traJ gene, albeit in a Dam-independent fashion. Hence, the decreased FinP RNA content found in Dam - hosts of S. enterica appears to result from H-NS-mediated repression of finP transcription. | es |
dc.description.sponsorship | Ministerio de Educación y Ciencia BIO2001-232-CO2-02 BIO2004-3455-CO2-02 | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | American Society for Microbiology | es |
dc.relation.ispartof | Journal of Bacteriology, 187 (16), 5691-5699. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Regulation of finP transcription by DNA adenine methylation in the virulence plasmid of Salmonella enterica | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Genética | es |
dc.relation.projectID | BIO2001-232-CO2-02 | es |
dc.relation.projectID | BIO2004-3455-CO2-02 | es |
dc.relation.publisherversion | http://dx.doi.org/10.1128/JB.187.16.5691-5699.2005 | es |
dc.identifier.doi | 10.1128/JB.187.16.5691-5699.2005 | es |
idus.format.extent | 9 p. | es |
dc.journaltitle | Journal of Bacteriology | es |
dc.publication.volumen | 187 | es |
dc.publication.issue | 16 | es |
dc.publication.initialPage | 5691 | es |
dc.publication.endPage | 5699 | es |
dc.contributor.funder | Ministerio de Educación y Ciencia (MEC). España | |