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Regulation of finP transcription by DNA adenine methylation in the virulence plasmid of Salmonella enterica

Opened Access Regulation of finP transcription by DNA adenine methylation in the virulence plasmid of Salmonella enterica

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Autor: Camacho Fernández, Eva María
Serna, Ana
Madrid, Cristina
Marqués Martín, Silvia
Fernández, Raúl
de la Cruz, Fernando
Juárez, Antonio
Casadesús Pursals, Josep
Departamento: Universidad de Sevilla. Departamento de Genética
Fecha: 2005-08
Publicado en: Journal of Bacteriology, 187 (16), 5691-5699.
Tipo de documento: Artículo
Resumen: DNA adenine methylase (Dam -) mutants of Salmonella enterica serovar Typhimurium contain reduced levels of FinP RNA encoded on the virulence plasmid. Dam methylation appears to regulate finP transcription, rather than FinP RNA stability or turnover. The finP promoter includes canonical -10 and -35 modules and depends on the σ 70 factor. Regulation of finP transcription by Dam methylation does not require DNA sequences upstream from the -35 module, indicating that Dam acts at the promoter itself or downstream. Unexpectedly, a GATC site overlapping with the -10 module is likewise dispensable for Dam-mediated regulation. These observations indicate that Dam methylation regulates finP transcription indirectly and suggest the involvement of a host factor(s) responsive to the Dam methylation state of the cell. We provide evidence that one such factor is the nucleoid protein H-NS, which acts as a repressor of finP transcription in a Dam - background. H-NS also restrains transcription of the ...
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Cita: Camacho Fernández, E.M., Serna, A., Madrid, C., Marqués Martín, S., Fernández, R., de la Cruz, F.,...,Casadesús Pursals, J. (2005). Regulation of finP transcription by DNA adenine methylation in the virulence plasmid of Salmonella enterica. Journal of Bacteriology, 187 (16), 5691-5699.
Tamaño: 244.1Kb
Formato: PDF

URI: http://hdl.handle.net/11441/62931

DOI: 10.1128/JB.187.16.5691-5699.2005

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