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NtcA-regulated heterocyst differentiation genes hetC and devB from Anabaena sp. strain PCC 7120 exhibit a similar tandem promoter arrangement

Opened Access NtcA-regulated heterocyst differentiation genes hetC and devB from Anabaena sp. strain PCC 7120 exhibit a similar tandem promoter arrangement

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Autor: Muro Pastor, María Isabel
Flores García, Enrique
Herrero Moreno, Antonia
Departamento: Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular
Fecha: 2009
Publicado en: Journal of Bacteriology, 191 (18), 5765-5774.
Tipo de documento: Artículo
Resumen: Transcription of the hetC gene, whose product is required for heterocyst differentiation, takes place from a long promoter region that includes the previously described HetR-independent, NtcA-activated promoter producing transcripts with a 5′ end corresponding to position -571 with respect to the translational start site of hetC. Northern blot analysis indicated that the accumulation of hetC transcripts depends on HetR, and a second transcriptional start site located at position -293 that leads to NtcA-dependent, HetR-dependent inducible transcription of hetC was identified. Upon nitrogen stepdown, expression of a PhetC::gfp fusion was transiently induced in specific cells that were differentiating into heterocysts, both when the whole promoter region (containing transcription start points -571 and -293) or a short version (containing only the transcription start point -293) was used. Expression of hetC from the -293 position was delayed in a strain bearing a deleted promoter region l...
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Cita: Muro Pastor, M.I., Flores García, E. y Herrero, A. (2009). NtcA-regulated heterocyst differentiation genes hetC and devB from Anabaena sp. strain PCC 7120 exhibit a similar tandem promoter arrangement. Journal of Bacteriology, 191 (18), 5765-5774.
Tamaño: 1.397Mb
Formato: PDF

URI: http://hdl.handle.net/11441/62905

DOI: 10.1128/JB.00616-09

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