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A major facilitator superfamily protein, HepP, is involved in formation of the heterocyst envelope polysaccharide in the cyanobacterium Anabaena sp. Strain PCC 7120

Opened Access A major facilitator superfamily protein, HepP, is involved in formation of the heterocyst envelope polysaccharide in the cyanobacterium Anabaena sp. Strain PCC 7120

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Autor: López Igual, Rocío
Lechno Yossef, Sigal
Fang, Qing
Herrero Moreno, Antonia
Flores García, Enrique
Wolk, C. Peter
Departamento: Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular
Fecha: 2012
Publicado en: Journal of Bacteriology, 194 (17), 4677-4687.
Tipo de documento: Artículo
Resumen: Some filamentous cyanobacteria such as Anabaena sp. strain PCC 7120 produce cells, termed heterocysts, specialized in nitrogen fixation. Heterocysts bear a thick envelope containing an inner layer of glycolipids and an outer layer of polysaccharide that restrict the diffusion of air(including O2) into the heterocyst. Anabaena sp. mutants impaired in production of either of those layers show a Fox- phenotype (requiring fixed nitrogen for growth under oxic conditions). We have characterized a set of transposon-induced Fox- mutants in which transposon Tn5-1063 was inserted into the Anabaena sp. chromosome open reading frame all1711 which encodes a predicted membrane protein that belongs to the major facilitator superfamily (MFS). These mutants showed higher nitrogenase activities under anoxic than under oxic conditions and altered sucrose uptake. Electron microscopy and alcian blue staining showed a lack of the heterocyst envelope polysaccharide (Hep) layer. Northern blot and primer exte...
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Cita: López Igual, o., Lechno Yossef, S., Fang, Q., Herrero, A., Flores García, E. y Wolk, C.P. (2012). A major facilitator superfamily protein, HepP, is involved in formation of the heterocyst envelope polysaccharide in the cyanobacterium Anabaena sp. Strain PCC 7120. Journal of Bacteriology, 194 (17), 4677-4687.
Tamaño: 3.615Mb
Formato: PDF

URI: http://hdl.handle.net/11441/62898

DOI: 10.1128/JB.00489-12

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