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Selection of small-colony variants of salmonella enterica serovar typhimurium in nonphagocytic eucaryotic cells

Opened Access Selection of small-colony variants of salmonella enterica serovar typhimurium in nonphagocytic eucaryotic cells

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Autor: A. Cano, David
Pucciarelli, María Graciela
Martínez Moya, Marina
Casadesús Pursals, Josep
García del Portillo, Francisco
Departamento: Universidad de Sevilla. Departamento de Genética
Fecha: 2003-07
Publicado en: Infection and Immunity, 71 (1), 3690-3698.
Tipo de documento: Artículo
Resumen: Salmonella enterica strains are enteropathogenic bacteria that survive and proliferate within vacuolar compartments of epithelial and phagocytic cells. Recently, it has been reported that fibroblast cells are capable of restricting S. enterica serovar Typhimurium intracellular growth. Here, we show that prolonged residence of bacteria in the intracellular environment of fibroblasts results in the appearance of genetically stable small-colony variants (SCV). A total of 103 SCV isolates, obtained from four independent infections, were subjected to phenotypic analysis. The following phenotypes were observed: (i) δ-aminolevulinic acid auxotrophy; (ii) requirement for acetate or succinate for growth in glucose minimal medium; (iii) auxotrophy for aromatic amino acids; and (iv) reduced growth rate under aerobic conditions not linked to nutrient auxotrophy. The exact mutations responsible for the SCV phenotype in three representative isolates were mapped in the lpd, hemL, and aroD genes, whi...
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Cita: A. Cano, D., Pucciarelli, M.G., Martínez Moya, M., Casadesús Pursals, J. y García del Portillo, F. (2003). Selection of small-colony variants of salmonella enterica serovar typhimurium in nonphagocytic eucaryotic cells. Infection and Immunity, 71 (1), 3690-3698.
Tamaño: 133.6Kb
Formato: PDF

URI: http://hdl.handle.net/11441/62693

DOI: 10.1128/IAI.71.7.3690-3698.2003

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