dc.creator | Prado Velasco, José Félix | es |
dc.creator | Aguilera López, Andrés | es |
dc.date.accessioned | 2017-07-05T14:17:17Z | |
dc.date.available | 2017-07-05T14:17:17Z | |
dc.date.issued | 2005 | |
dc.identifier.citation | Prado Velasco, J.F. y Aguilera López, A. (2005). Partial depletion of histone H4 increases homologous recombination-mediated genetic instability. Molecular and Cellular Biology, 25 (4), 1526-1536. | |
dc.identifier.issn | 0270-7306 | es |
dc.identifier.uri | http://hdl.handle.net/11441/62056 | |
dc.description.abstract | DNA replication can be a source of genetic instability. Given the tight connection between DNA replication
and nucleosome assembly, we analyzed the effect of a partial depletion of histone H4 on genetic instability
mediated by homologous recombination. A Saccharomyces cerevisiae strain was constructed in which the
expression of histone H4 was driven by the regulated tet promoter. In agreement with defective nucleosome
assembly, partial depletion of histone H4 led to subtle changes in plasmid superhelical density and chromatin
sensitivity to micrococcal nuclease. Under these conditions, homologous recombination between ectopic DNA
sequences was increased 20-fold above the wild-type levels. This hyperrecombination was not associated with
either defective repair or transcription but with an accumulation of recombinogenic DNA lesions during the S
and G2/M phases, as determined by an increase in the proportion of budded cells containing Rad52-yellow
fluorescent protein foci. Consistently, partial depletion of histone H4 caused a delay during the S and G2/M
phases. Our results suggest that histone deposition defects lead to the formation of recombinogenic DNA
structures during replication that increase genomic instability. | es |
dc.description.sponsorship | Ministerio de Ciencia y Tecnología BMC2000-0439 SAF2003-00204 | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | American Society for Microbiology | es |
dc.relation.ispartof | Molecular and Cellular Biology, 25 (4), 1526-1536. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Partial depletion of histone H4 increases homologous recombination-mediated genetic instability | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Genética | es |
dc.relation.projectID | BMC2000-0439 | es |
dc.relation.projectID | SAF2003-00204 | es |
dc.relation.publisherversion | http://dx.doi.org/10.1128/MCB.25.4.1526–1536.2005 | es |
dc.identifier.doi | 10.1128/MCB.25.4.1526–1536.2005 | es |
idus.format.extent | 11 p. | es |
dc.journaltitle | Molecular and Cellular Biology | es |
dc.publication.volumen | 25 | es |
dc.publication.issue | 4 | es |
dc.publication.initialPage | 1526 | es |
dc.publication.endPage | 1536 | es |
dc.identifier.sisius | 6655174 | es |
dc.contributor.funder | Ministerio de Ciencia y Tecnología (MCYT). España | |