Repositorio de producción científica de la Universidad de Sevilla

Characterization of Nuclear Microsatellite Markers for Rumex bucephalophorus (Polygonaceae) Using 454 Sequencing

 

Advanced Search
 
Opened Access Characterization of Nuclear Microsatellite Markers for Rumex bucephalophorus (Polygonaceae) Using 454 Sequencing
Cites

Show item statistics
Icon
Export to
Author: Viruel, Juan
Ortiz Ballesteros, Pedro Luis
Arista Palmero, Montserrat
Talavera Solís, María Manuela
Department: Universidad de Sevilla. Departamento de Biología Vegetal y Ecología
Date: 2015
Published in: Applications in Plant Sciences, 3 (12), 1-4.
Document type: Article
Abstract: Premise of the study: Nuclear microsatellite markers were developed in Rumex bucephalophorus subsp. canariensis (Polygonaceae) to investigate its genetic diversity and structure. Methods and Results: Sixteen polymorphic microsatellite markers were obtained using 454 next-generation sequencing with di-, tri-, and tetranucleotide repeats. The average number of alleles was 5.688 and 3.813 for R. bucephalophorus subsp. canariensis var. canariensis and var. fruticescens, respectively. Slightly higher levels of mean genetic diversity were found in var. canariensis (expected heterozygosity = 0.600) than in var. fruticescens (expected heterozygosity = 0.514). Cross-amplifications in related taxa within R. bucephalophorus showed good amplification and polymorphic patterns. Conclusions: These 16 novel nuclear microsatellite markers are the first in the genus Rumex and may serve as valuable tools to carry out studies on genetic diversity and structure as well as progeny studies.
Cite: Viruel, J., Ortiz Ballesteros, P.L., Arista Palmero, M. y Talavera Solís, M.M. (2015). Characterization of Nuclear Microsatellite Markers for Rumex bucephalophorus (Polygonaceae) Using 454 Sequencing. Applications in Plant Sciences, 3 (12), 1-4.
Size: 503.9Kb
Format: PDF

URI: http://hdl.handle.net/11441/59413

DOI: 10.3732/apps.1500088

This work is under a Creative Commons License: 
Attribution-NonCommercial-NoDerivatives 4.0 Internacional

This item appears in the following Collection(s)