Show simple item record

dc.creatorDelgado Ramos, Lidiaes
dc.creatorMarcos Rodríguez, Ana Teresaes
dc.creatorRamos Guelfo, María Saludes
dc.creatorSánchez Barrionuevo, Leyrees
dc.creatorChávez de Diego, Sebastiánes
dc.creatorCánovas López, Davides
dc.date.accessioned2017-03-08T14:18:23Z
dc.date.available2017-03-08T14:18:23Z
dc.date.issued2014
dc.identifier.citationDelgado Ramos, L., Marcos Rodríguez, A.T., Ramos Guelfo, M.S., Sánchez Barrionuevo, L., Chávez de Diego, S. y Cánovas López, D. (2014). Flow Cytometry of Microencapsulated Colonies for Genetics Analysis of Filamentous Fungi. G3: Genes, Genomes, Genetics, 4 (11), 2271-2278.
dc.identifier.issn2160-1836es
dc.identifier.urihttp://hdl.handle.net/11441/55587
dc.description.abstractThe analysis of filamentous fungi by flow cytometry has been impossible to date due to their filamentous nature and size. In this work, we have developed a method that combines single-spore microencapsulation and large-particle flow cytometry as a powerful alternative for the genetic analysis of filamentous fungi. Individual spores were embedded in monodisperse alginate microparticles and incubated in the appropriate conditions. Growth could be monitored by light or fluorescent microscopy and Complex Object Parametric Analyzer and Sorter large-particle flow cytometry. Microencapsulated Trichoderma and Aspergillus spores could germinate and grow inside the alginate capsules. Growth tests revealed that auxotrophic mutants required the appropriate nutrients and that pyrithiamine and glufosinate halted fungal growth of sensitive but not resistant strains. We used an Aspergillus nidulans, thermosensitive mutant in the cell-cycle regulator gene nimXCDK1 as proof-of-concept to the detection and identification of genetic phenotypes. Sorting of the microparticles containing the clonal fungal mycelia proved the power of this method to perform positive and/or negative selection during genetic screenings.es
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherGenetics Society of America:es
dc.relation.ispartofG3: Genes, Genomes, Genetics, 4 (11), 2271-2278.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectfilamentous fungies
dc.subjectAspergilluses
dc.subjectflow cytometryes
dc.subjectmicroencapsulationes
dc.subjectgenetic analysises
dc.titleFlow Cytometry of Microencapsulated Colonies for Genetics Analysis of Filamentous Fungies
dc.typeinfo:eu-repo/semantics/articlees
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessrightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Genéticaes
dc.relation.publisherversionhttp://dx.doi.org/10.1534/g3.114.014357es
dc.identifier.doi10.1534/g3.114.014357es
idus.format.extent8 p.es
dc.journaltitleG3: Genes, Genomes, Geneticses
dc.publication.volumen4es
dc.publication.issue11es
dc.publication.initialPage2271es
dc.publication.endPage2278es

FilesSizeFormatViewDescription
pub152271.pdf1.584MbIcon   [PDF] View/Open  

This item appears in the following collection(s)

Show simple item record

Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Except where otherwise noted, this item's license is described as: Attribution-NonCommercial-NoDerivatives 4.0 Internacional