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Opening the "black box" of nodD3, nodD4 and nodD5 genes of Rhizobium tropici strain CIAT 899

 

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dc.creator Cerro Sánchez, Pablo del es
dc.creator Rolla-Santos, Amanda Alves Paiva es
dc.creator Gomes, Douglas Fabiano es
dc.creator Berquó Marks, Bettina es
dc.creator Espuny Gómez, María del Rosario es
dc.creator Rodríguez Carvajal, Miguel Ángel es
dc.creator Soria Díaz, María Eugenia es
dc.creator Shigueyoshi Nakatani, André es
dc.creator Hungria, Mariangela es
dc.creator Ollero Márquez, Francisco Javier es
dc.creator Megías Guijo, Manuel es
dc.date.accessioned 2016-10-25T10:51:10Z
dc.date.available 2016-10-25T10:51:10Z
dc.date.issued 2015
dc.identifier.citation Cerro Sánchez, P.d., Rolla-Santos, A.A.P., Gomes, D.F., Berquó Marks, B., Espuny Gómez, M.d.R., Rodríguez Carvajal, M.Á.,...,Megías Guijo, M. (2015). Opening the "black box" of nodD3, nodD4 and nodD5 genes of Rhizobium tropici strain CIAT 899. BMC Genomics, 16 (1), 1-10.
dc.identifier.issn 1471-2164 es
dc.identifier.uri http://hdl.handle.net/11441/48089
dc.description.abstract Background: Transcription of nodulation genes in rhizobial species is orchestrated by the regulatory nodD gene. Rhizobium tropici strain CIAT 899 is an intriguing species in possessing features such as broad host range, high tolerance of abiotic stresses and, especially, by carrying the highest known number of nodD genes-five-and the greatest diversity of Nod factors (lipochitooligosaccharides, LCOs). Here we shed light on the roles of the multiple nodD genes of CIAT 899 by reporting, for the first time, results obtained with nodD3, nodD4 and nodD5 mutants. Methods: The three nodD mutants were built by insertion of Ω interposon. Nod factors were purified and identified by LC-MS/MS analyses. In addition, nodD1 and nodC relative gene expressions were measured by quantitative RT-PCR in the wt and derivative mutant strains. Phenotypic traits such as exopolysaccharide (EPS), lipopolysaccharide (LPS), swimming and swarming motilities, biofilm formation and indole acetid acid (IAA) production were also perfomed. All these experiments were carried out in presence of both inducers of CIAT 899, apigenin and salt. Finally, nodulation assays were evaluated in up to six different legumes, including common bean (Phaseolus vulgaris L.). Results: Phenotypic and symbiotic properties, Nod factors and gene expression of nodD3, nodD4 and nodD5 mutants were compared with those of the wild-type (WT) CIAT 899, both in the presence and in the absence of the nod-gene-inducing molecule apigenin and of saline stress. No differences between the mutants and the WT were observed in exopolysaccharide (EPS) and lipopolysaccharide (LPS) profiles, motility, indole acetic acid (IAA) synthesis or biofilm production, either in the presence, or in the absence of inducers. Nodulation studies demonstrated the most complex regulatory system described so far, requiring from one (Leucaena leucocephala, Lotus burtii) to four (Lotus japonicus) nodD genes. Up to 38 different structures of Nod factors were detected, being higher under salt stress, except for the nodD5 mutant; in addition, a high number of structures was synthesized by the nodD4 mutant in the absence of any inducer. Probable activator (nodD3 and nodD5) or repressor roles (nodD4), possibly via nodD1 and/or nodD2, were attributed to the three nodD genes. Expression of nodC, nodD1 and each nodD studied by RT-qPCR confirmed that nodD3 is an activator of nodD1, both in the presence of apigenin and salt stress. In contrast, nodD4 might be an inducer with apigenin and a repressor under saline stress, whereas nodD5 was an inducer under both conditions. Conclusions: We report for R. tropici CIAT 899 the most complex model of regulation of nodulation genes described so far. Five nodD genes performed different roles depending on the host plant and the inducing environment. Nodulation required from one to four nodD genes, depending on the host legume. nodD3 and nodD5 were identified as activators of the nodD1 gene, whereas, for the first time, it was shown that a regulatory nodD gene-nodD4-might act as repressor or inducer, depending on the inducing environment, giving support to the hypothesis that nodD roles go beyond nodulation, in terms of responses to abiotic stresses es
dc.format application/pdf es
dc.language.iso eng es
dc.publisher BioMed Central es
dc.relation.ispartof BMC Genomics, 16 (1), 1-10.
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 Internacional *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/ *
dc.subject Biological nitrogen fixation es
dc.subject LCO es
dc.subject Nod factors es
dc.subject NodD gene es
dc.subject Symbiosis es
dc.title Opening the "black box" of nodD3, nodD4 and nodD5 genes of Rhizobium tropici strain CIAT 899 es
dc.type info:eu-repo/semantics/article es
dc.type.version info:eu-repo/semantics/publishedVersion es
dc.rights.accessrights info:eu-repo/semantics/openAccess es
dc.contributor.affiliation Universidad de Sevilla. Departamento de Microbiología es
dc.relation.publisherversion 10.1186/s12864-015-2033-z es
dc.identifier.doi 10.1186/s12864-015-2033-z es
idus.format.extent 10 p. es
dc.journaltitle BMC Genomics es
dc.publication.volumen 16 es
dc.publication.issue 1 es
dc.publication.initialPage 1 es
dc.publication.endPage 10 es
dc.identifier.idus https://idus.us.es/xmlui/handle/11441/48089
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