dc.creator | Prado Gotor, Rafael | es |
dc.creator | Grueso Molina, Elia María | es |
dc.date.accessioned | 2016-10-25T10:48:25Z | |
dc.date.available | 2016-10-25T10:48:25Z | |
dc.date.issued | 2014 | |
dc.identifier.citation | Prado Gotor, R. y Grueso Molina, E.M. (2014). Noncovalent interactions of tiopronin-protected gold nanoparticles with DNA: two methods to quantify free energy of binding. The Scientific World Journal, 2014, 143645-1-143645-9. | |
dc.identifier.uri | http://hdl.handle.net/11441/48086 | |
dc.description.abstract | The binding of gold nanoparticles capped with N-(2-mercaptopropionyl)glycine (Au@tiopronin) with double-stranded DNA has
been investigated and quantified in terms of free energies by using two different approaches. The first approach follows the DNA
conformational changes induced by gold nanoparticles using the CD technique. The second methodology consists in the use of
pyrene-1-carboxaldehyde as a fluorescent probe. This second procedure implies the determination of the “true” free energy of
binding of the probe with DNA, after corrections through solubility measurements. Working at different salt concentrations, the
nonelectrostatic and electrostatic components of the binding free energy have been separated.The results obtained revealed that the
binding is of nonelectrostatic character, fundamentally.The procedure used in this work could be extended to quantify the binding
affinity of other AuNPs/DNA systems. | es |
dc.description.sponsorship | Junta de Andalucia, Ministerio de Educación y Ciencia DIGYT CTQ2008-0008/BQU | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.relation.ispartof | The Scientific World Journal, 2014, 143645-1-143645-9. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Noncovalent interactions of tiopronin-protected gold nanoparticles with DNA: two methods to quantify free energy of binding | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Química Física | es |
dc.relation.projectID | DIGYT CTQ2008-0008/BQU | es |
dc.relation.publisherversion | https://www.hindawi.com/journals/tswj/2014/143645/ | es |
dc.identifier.doi | 10.1155/2014/143645 | es |
idus.format.extent | 10 p. | es |
dc.journaltitle | The Scientific World Journal | es |
dc.publication.volumen | 2014 | es |
dc.publication.initialPage | 143645-1 | es |
dc.publication.endPage | 143645-9 | es |
dc.identifier.idus | https://idus.us.es/xmlui/handle/11441/48086 | |
dc.contributor.funder | Ministerio de Educación y Ciencia (MEC). España | |