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Isolation and characterization of salt-sensitive mutants of the moderate halophile Halomonas elongata and cloning of the ectoine synthesis genes

Opened Access Isolation and characterization of salt-sensitive mutants of the moderate halophile Halomonas elongata and cloning of the ectoine synthesis genes

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Autor: Cánovas López, David
Vargas, C.
Iglesias Guerra, Fernando
Csonka, Laszlo N.
Rhodes, David R.
Ventosa Ucero, Antonio
Nieto Gutiérrez, Joaquín José
Departamento: Universidad de Sevilla. Departamento de Microbiología y Parasitología
Fecha: 1997
Publicado en: Journal of Biological Chemistry, 272 (41), 25794-25801.
Tipo de documento: Artículo
Resumen: The moderate halophile Halomonas elongata Deustche Sommlung fur Mikroorganismen 3043 accumulated ectoine, hydroxyectoine, glutamate, and glutamine in response to osmotic stress (3 M NaCl). Two Tn1732-induced mutants, CHR62 and CHR63, that were severely affected in their salt tolerance were isolated. Mutant CHR62 could not grow above 0.75 M NaCl, and CHR63 did not grow above 1.5 M NaCl. These mutants did not synthesize ectoine but accumulated ectoine precursors, as shown by 13C NMR and mass spectroscopy. Mutant CHR62 accumulated low levels of diaminobutyric acid, and mutant CHR63 accumulated high concentrations of N-γ-acetyl-diaminobutyric acid. These results suggest that strain CHR62 could be defective in the gene for diaminobutyric acid acetyltransferase (ectB), and strain CHR63 could be defective in the gene for the ectoine synthase (ectC). Salt sensitivity of the mutants at 1.5-2.5 M NaCl could be partially corrected by cytoplasmic extracts of the wild-type strain, containing ectoi...
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Cita: Cánovas, D., Vargas, C., Iglesias Guerra, F., Csonka, L.N., Rhodes, D.R., Ventosa Ucero, A. y Nieto Gutiérrez, J.J. (1997). Isolation and characterization of salt-sensitive mutants of the moderate halophile Halomonas elongata and cloning of the ectoine synthesis genes. Journal of Biological Chemistry, 272 (41), 25794-25801.
Tamaño: 315.3Kb
Formato: PDF

URI: http://hdl.handle.net/11441/42504

DOI: 10.1074/jbc.272.41.25794

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