Repositorio de producción científica de la Universidad de Sevilla

Toward the Assessment of Food Toxicity for Celiac Patients: Characterization of Monoclonal Antibodies to a Main Immunogenic Gluten Peptide

Opened Access Toward the Assessment of Food Toxicity for Celiac Patients: Characterization of Monoclonal Antibodies to a Main Immunogenic Gluten Peptide

Citas

buscar en

Estadísticas
Icon
Exportar a
Autor: Morón Flores, Belén
Bethune, Michael T.
Comino Montilla, Isabel María
Manyani, Hamid
Ferragud, Marina
López, Manuel Carlos
Cebolla, Ángel
Khosla, Chaitan
Sousa Martín, Carolina
Departamento: Universidad de Sevilla. Departamento de Microbiología y Parasitología
Fecha: 2008
Publicado en: PLoS One, 3 (5), e2294-.
Tipo de documento: Artículo
Resumen: Background and Aims: Celiac disease is a permanent intolerance to gluten prolamins from wheat, barley, rye and, in some patients, oats. Partially digested gluten peptides produced in the digestive tract cause inflammation of the small intestine. High throughput, immune-based assays using monoclonal antibodies specific for these immunotoxic peptides would facilitate their detection in food and enable monitoring of their enzymatic detoxification. Two monoclonal antibodies, G12 and A1, were developed against a highly immunotoxic 33-mer peptide. The potential of each antibody for quantifying food toxicity for celiac patients was studied. Methods: Epitope preferences of G12 and A1 antibodies were determined by ELISA with gluten-derived peptide variants of recombinant, synthetic or enzymatic origin. Results: The recognition sequences of G12 and A1 antibodies were hexameric and heptameric epitopes, respectively. Although G12 affinity for the 33-mer was superior to A1, the sensitivity f...
[Ver más]
Cita: Morón, B., Bethune, M.T., Comino Montilla, I.M., Manyani, H., Ferragud, M., López, M.C.,...,Sousa Martín, C. (2008). Toward the Assessment of Food Toxicity for Celiac Patients: Characterization of Monoclonal Antibodies to a Main Immunogenic Gluten Peptide. PLoS One, 3 (5), e2294-.
Tamaño: 800.1Kb
Formato: PDF

URI: http://hdl.handle.net/11441/41963

DOI: http://dx.doi.org/10.1371/journal.pone.0002294

Mostrar el registro completo del ítem


Esta obra está bajo una Licencia Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 Internacional

Este registro aparece en las siguientes colecciones