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Microscopic and transcriptome analyses of early colonization of tomato roots by "Trichoderma harzianum".

 

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dc.creator Chacón, Mariola R. es
dc.creator Rodríguez Galán, Olga es
dc.creator Benítez Fernández, Concepción Tahía es
dc.creator Sousa, Sonia es
dc.creator Rey, Manuel es
dc.creator Llobell González, Antonio es
dc.creator Delgado Jarana, Jesús es
dc.date.accessioned 2016-05-25T11:17:20Z
dc.date.available 2016-05-25T11:17:20Z
dc.date.issued 2007
dc.identifier.citation Chacón, M.R., Rodríguez Galán, O., Benítez Fernández, C.T., Sousa, S., Rey, M., Llobell González, A. y Delgado Jarana, J. (2007). Microscopic and transcriptome analyses of early colonization of tomato roots by "Trichoderma harzianum".. International microbiology: official journal of the Spanish Society for Microbiology, 10 (1), 19-27.
dc.identifier.issn 1139-6709 es
dc.identifier.uri http://hdl.handle.net/11441/41597
dc.description.abstract The capacity of the fungus Trichoderma harzianum CECT 2413 to colonize roots and stimulate plant growth was analyzed. Tobacco seedlings (Nicotiana benthamiana) transferred to Petri dishes inoculated with T. harzianum conidia showed increased plant fresh weight (140%) and foliar area (300%), as well as the proliferation of secondary roots (300%) and true leaves (140%). The interaction between strain CECT 2413 and the tomato-root system was also studied during the early stages of root colonization by the fungus. When T. harzianum conidia were inoculated into the liquid medium of hydroponically grown tomato plants (Lycopersicum esculentum), profuse adhesion of hyphae to the plant roots as well as colonization of the root epidermis and cortex were observed. Confocal microscopy of a T. harzianum transformant that expressed the green fluorescent protein (GFP) revealed intercellular hyphal growth and the formation of plant-induced papilla-like hyphal tips. Analysis of the T. harzianum-tomato interaction in soil indicated that the contact between T. harzianum and the roots persisted over a long period of time. This interaction was characterized by the presence of yeast-like cells, a novel and previously undescribed developmental change. To study the molecular mechanism underlying fungal ability to colonize the tomato-root system, the T. harzianum transcriptome was analyzed during the early stages of the plant-fungus interaction. The expression of fungal genes related to redox reactions, lipid metabolism, detoxification, and sugar or amino-acid transport increased when T. harzianum colonized tomato roots. These observations are similar to those regarding the interactions of mycorrhiza and pathogenic fungi with plants. es
dc.description.sponsorship Comisión Europea QLK3-CT-2002-02032 es
dc.description.sponsorship Ministerio de Ciencia y Tecnologia AGL2000-0524 and BIO2003-03679 es
dc.description.sponsorship Junta de Andalucía PAI CVI-107 es
dc.format application/pdf es
dc.language.iso eng es
dc.publisher Institut d´Estudis Catalans es
dc.relation.ispartof International microbiology: official journal of the Spanish Society for Microbiology, 10 (1), 19-27.
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 Internacional *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/ *
dc.subject Trichoderma harzianum es
dc.subject plant–fungus interaction es
dc.subject tomato es
dc.subject tobacco es
dc.subject mycorrhiza es
dc.subject gene expression es
dc.title Microscopic and transcriptome analyses of early colonization of tomato roots by "Trichoderma harzianum". es
dc.type info:eu-repo/semantics/article es
dc.type.version info:eu-repo/semantics/publishedVersion es
dc.rights.accessrights info:eu-repo/semantics/openAccess es
dc.contributor.affiliation Universidad de Sevilla. Departamento de Genética es
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP5/QLK3-CT-2002-02032 es
dc.relation.projectID AGL2000-0524 es
dc.relation.projectID BIO2003-03679 es
dc.relation.projectID PAI CVI-107 es
dc.relation.publisherversion http://www.im.microbios.org/1001/1001019.pdf es
dc.relation.publisherversion http://dx.doi.org/10.2436/20.1501.01.4
dc.identifier.doi 10.2436/20.1501.01.4
idus.format.extent 9 p. es
dc.journaltitle International microbiology: official journal of the Spanish Society for Microbiology es
dc.publication.volumen 10 es
dc.publication.issue 1 es
dc.publication.initialPage 19 es
dc.publication.endPage 27 es
dc.identifier.idus https://idus.us.es/xmlui/handle/11441/41597
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