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Confonnations in crystals and solutions of d(CACGTG), d(CCGCGG) and d(GGCGCC) studied by vibrational spectroscopy

 

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Opened Access Confonnations in crystals and solutions of d(CACGTG), d(CCGCGG) and d(GGCGCC) studied by vibrational spectroscopy
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Author: Urpi, L.
Ridouxl, J.P.
Liquier, J.
Verdaguer, N.
Fita. I.
Subirana, J.A.
Iglesias Guerra, Fernando
Huynh-Dinh, T.
Igolen, J.
Taillandier, E.
Department: Universidad de Sevilla. Departamento de Química Orgánica y Farmacéutica
Date: 1989
Published in: Nucleic Acids Research, 17 (16), 6669-6680.
Document type: Article
Abstract: Crystals of self camplementary DMA hexamers dCCACGTG>, dCCCGCGG> and d<GGCGCC> were grown bf vapour dlffuslon technlque and studled by mlcroRaman and mlcroiR spectroscop es. The ollgonucleotldes were studled ln parallel ln solutlon by vlbratlonal spectroscopy. A B->Z transltlon was detected by Raman spectroscopy cl.lrlng the crystalllzatlon procese for dCCACGTG>. Vlbratlonal spectroscopy shows that the dCGGCGCC> crystals adopt a B geametry. On the contrary the d<CCGCGG> sequence whlch ls shown to be able to undergo ln solutlon or ln fllms qulte easlly the B->Z transltlon, remalns trapped ln crystals ln a geametry whlch may correspond to an lntermedlate conformatlon often proposed ln modele of the B->Z transltlon. The crystals used ln thls study were characterlzed by X-ray dlffractlon. The unlt cell and space group have been determlned.
Cite: Urpi, L., Ridouxl, J.P., Liquier, J., Verdaguer, N., Fita. I., , Subirana, J.A.,...,Taillandier, E. (1989). Confonnations in crystals and solutions of d(CACGTG), d(CCGCGG) and d(GGCGCC) studied by vibrational spectroscopy. Nucleic Acids Research, 17 (16), 6669-6680.
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URI: http://hdl.handle.net/11441/41403

DOI: 10.1093/nar/17.16.6669

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