Repositorio de producción científica de la Universidad de Sevilla

Genetic Variability among ampC Genes from Acinetobacter Genomic Species 3

Opened Access Genetic Variability among ampC Genes from Acinetobacter Genomic Species 3

Citas

buscar en

Estadísticas
Icon
Exportar a
Autor: Beceiro Casas, Alejandro
Pérez, Astrid
Fernández-Cuenca, Felipe
Martínez-Martínez, Luis
Pascual Hernández, Álvaro
Vila, Jordi
Rodríguez-Baño, Jesús
Cisneros, José Miguel
Pachón, Jerónimo
Bou, Germán
Departamento: Universidad de Sevilla. Departamento de Medicina
Fecha: 2009-03
Publicado en: Antimicrobial Agents and Chemotherapy, 53(3), 1177–1184
Tipo de documento: Artículo
Resumen: As a part of a nationwide study in Spain, 15 clinical isolates of Acinetobacter genomic species 3 (AG3) were analyzed. The main objective of the study was to characterize the ampC genes from these isolates and to determine their involvement in B-lactam resistance in AG3. The 15 AG3 isolates showed different profiles of resistance to ampicillin (range of MICs, 12 to >256 μg/ml). Nucleotide sequencing of the 15 ampC genes yielded 12 new AmpC enzymes (ADC-12 to ADC-23). The 12 AG3 enzymes showed 93.7 to 96.1% amino acid identity with respect to the AmpC enzyme from Acinetobacter baumannii (ADC-1 enzyme). Eight out of fifteen ampC genes were expressed in Escherichia coli cells under the control of a common promoter, and with the exception of one isolate (isolate 65, which showed lower B-lactam MICs), significant differences in overall B-lactam MICs for E. coli cells expressing AG3 ampC genes were not revealed. No significant differences in ampC gene expression in AG3 clinical isolates we...
[Ver más]
Tamaño: 767.1Kb
Formato: PDF

URI: http://hdl.handle.net/11441/41212

DOI: http://dx.doi.org/10.1128/aac.00485-08

Ver versión del editor

Mostrar el registro completo del ítem


Esta obra está bajo una Licencia Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 Internacional

Este registro aparece en las siguientes colecciones