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Effect of protein kinase a activity on the association of ADP-ribosylation factor 1 to Golgi membranes

Opened Access Effect of protein kinase a activity on the association of ADP-ribosylation factor 1 to Golgi membranes

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Autor: Martín Rubio, María Esther
Hidalgo Jiménez, Josefina
Rosa, José Luis
Crottet, Pascal
Velasco López, Ángel
Departamento: Universidad de Sevilla. Departamento de Biología Celular
Fecha: 2000
Publicado en: The Journal of Biological Chemistry, 275(25, Issue of June 23),19050–19059
Tipo de documento: Artículo
Resumen: The small GTP-binding protein ADP-ribosylation factor 1 (ARF1) is an essential component of the molecular machinery that catalyzes the formation of membranebound transport intermediates. By using an in vitro assay that reproduces recruitment of cytosolic proteins onto purified, high salt-washed Golgi membranes, we have analyzed the role of cAMP-dependent protein kinase A (PKA) on ARF1 incorporation. Addition to this assay of either pure catalytic subunits of PKA (C-PKA) or cAMP increased ARF1 binding. By contrast, ARF1 association was inhibited following C-PKA inactivation with either PKA inhibitory peptide or RIIa as well as after cytosol depletion of C-PKA. C-PKA also stimulated recruitment and activation of a recombinant form of human ARF1 in the absence of additional cytosolic components. The binding step could be dissociated from the activation reaction and found to be independent of guanine nucleotides and saturable. This step was stimulated by C-PKA in an ATP-...
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Tamaño: 12.68Mb
Formato: PDF

URI: http://hdl.handle.net/11441/40671

DOI: 10.1074/jbc.275.25.19050

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